Zöbisch H, Gaede W, Kretzschmar C
Landesveterinär- und Lebensmitteluntersuchungsamt Stendal.
Berl Munch Tierarztl Wochenschr. 1994 Mar;107(3):85-90.
Because of the intensive efforts required and the dissatisfactory sensitivity achieved with the traditional serological diagnostic techniques for Avian Encephalomyelitis, an ELISA was developed and tested to show the presence of antibodies in chicken serum. Using a multi-phase antigen preparation and optimizing the reagents and serum dilutions, a method was developed which shows considerable advantages in sensitivity, specificity, practicability and costs compared with the Embryo Serum Neutralization Test.
由于传统血清学诊断技术用于禽脑脊髓炎时需要付出巨大努力且灵敏度不尽人意，因此开发并测试了一种酶联免疫吸附测定法（ELISA），以检测鸡血清中抗体的存在。通过使用多阶段抗原制剂并优化试剂和血清稀释度，开发出了一种方法，该方法与胚胎血清中和试验相比，在灵敏度、特异性、实用性和成本方面显示出相当大的优势。
