利用 Illumina MiSeq 测序技术对松叶耳蕨、耳叶牛皮消（杜鹃花科）微卫星标记的特征分析。
Characterization of microsatellite markers for pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing.
机构信息
Centre College, 600 West Walnut Street, Danville, Kentucky 40422 USA.
Department of Botany, University of Wyoming, 1000 E. University Ave., Laramie, Wyoming 82071 USA.
出版信息
Appl Plant Sci. 2014 Oct 29;2(11). doi: 10.3732/apps.1400072. eCollection 2014 Nov.
PREMISE OF THE STUDY
Pterospora andromedea (Ericaceae) is a mycoheterotrophic plant endemic to North America with a disjunct distribution. Eastern populations are in decline compared to western populations. Microsatellite loci will allow comparison of genetic diversity in endangered to nonthreatened populations. •
METHODS AND RESULTS
Illumina MiSeq sequencing resulted in development of 12 polymorphic microsatellite loci from 63 perfect microsatellite loci tested. One polymorphic locus was obtained from a traditional enrichment method. These 13 loci were screened across two western and two eastern populations. For western and eastern populations, respectively, number of alleles ranged from one to 10 and one to four, and observed heterozygosity ranged from 0.000 to 0.389 and 0.000 to 0.143. •
CONCLUSIONS
These are the first microsatellite loci developed for Pterospora. They will be useful in conservation efforts of the eastern populations and for examination of population genetic parameters at different geographic scales and comparison with mycorrhizal fungal hosts.
研究前提
翼核果（杜鹃花科）是一种北美特有且分布不连续的菌根异养植物。与西部种群相比，东部种群正在减少。微卫星基因座将允许比较濒危和非威胁种群的遗传多样性。
方法和结果
Illumina MiSeq 测序结果从 63 个完美微卫星基因座中开发出 12 个多态性微卫星基因座。一个多态性基因座是从传统的富集方法中获得的。这 13 个基因座在两个西部和两个东部种群中进行了筛选。西部和东部种群的等位基因数分别为 1 到 10 和 1 到 4，观察杂合度分别为 0.000 到 0.389 和 0.000 到 0.143。
结论
这些是为翼核果开发的第一批微卫星基因座。它们将有助于东部种群的保护工作，并用于在不同地理尺度上检查种群遗传参数，并与菌根真菌宿主进行比较。
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