Simmons Catherine L, Lamont Robert W, Shapcott Alison
Genecology Research Centre, Faculty of Science Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC 4558, Queensland, Australia.
Queensland Herbarium, Department of Science, Information Technology and Innovation, Toowong 4066, Queensland, Australia.
Appl Plant Sci. 2017 Dec 22;5(12). doi: 10.3732/apps.1700085. eCollection 2017 Dec.
The swamp orchid, (Orchidaceae), is nationally endangered due to illegal collection and habitat loss and fragmentation, resulting in a disjunct distribution in spring and coastal wetland ecotones along Australia's east coast. Polymorphic microsatellite markers were developed to study genetic diversity and population structure for conservation and restoration purposes.
Illumina HiSeq high-throughput sequencing was used to develop 15 nuclear microsatellite markers, including 10 polymorphic markers for . Polymorphism at each marker was evaluated using 90 individuals from four natural populations. The number of alleles per locus ranged from one to three, and the observed and expected heterozygosity varied from 0.036 to 0.944 and from 0.035 to 0.611, respectively. These markers transferred successfully to congener .
The microsatellite markers will be useful for revealing levels of genetic diversity and gene flow for and may inform future conservation efforts.
沼泽兰花（兰科）因非法采集、栖息地丧失和破碎化而在全国范围内濒临灭绝，导致其在澳大利亚东海岸的春季和沿海湿地生态交错带呈间断分布。为了保护和恢复的目的，开发了多态性微卫星标记来研究其遗传多样性和种群结构。
利用Illumina HiSeq高通量测序技术开发了15个核微卫星标记，其中包括10个针对[具体物种未提及]的多态性标记。使用来自四个自然种群的90个个体评估了每个标记的多态性。每个位点的等位基因数从1到3不等，观察到的杂合度和预期杂合度分别从0.036到0.944和从0.035到0.611不等。这些标记成功转移到同属物种[具体物种未提及]。
微卫星标记将有助于揭示[具体物种未提及]的遗传多样性水平和基因流，并可能为未来的保护工作提供参考。
