De Ji, Zhu Weidong, Liu Tianmeng, Wang Zhe, Zhong Yang
School of Life Sciences, Fudan University, Shanghai 200438, People's Republic of China; Science Faculty, Tibet University, Lhasa 850000, Tibet Autonomous Region, People's Republic of China.
Science Faculty, Tibet University, Lhasa 850000, Tibet Autonomous Region, People's Republic of China.
Appl Plant Sci. 2017 Mar 10;5(3). doi: 10.3732/apps.1600104. eCollection 2017 Mar.
(Ephedraceae), occurring in the Himalayan ranges, is an important plant species used in Tibetan medicine. Due to the lack of molecular markers to characterize genetic diversity, knowledge for conservation and uses of resources is limited; we therefore developed microsatellite markers for use in this species.
Using Illumina MiSeq sequencing technology, we developed 29 polymorphic microsatellite loci suitable for , of which 15 loci also showed polymorphisms in two related species, and . The average number of effective alleles per locus ranged from two to six. The observed and expected heterozygosity ranged from 0.23 to 0.83 and 0.44 to 0.86, respectively, in populations.
The developed 29 microsatellite markers are effective for the study of genetic structure and genetic diversity of , and 15 of these markers are suitable for related species.
麻黄科植物生长于喜马拉雅山脉，是藏药中一种重要的植物物种。由于缺乏用于表征遗传多样性的分子标记，关于该资源保护和利用的知识有限；因此，我们开发了微卫星标记用于该物种。
利用Illumina MiSeq测序技术，我们开发了29个适用于该物种的多态性微卫星位点，其中15个位点在两个相关麻黄属物种中也表现出多态性。每个位点的有效等位基因平均数在2到6之间。在该物种群体中，观察到的杂合度和预期杂合度分别在0.23至0.83和0.44至0.86之间。
开发的29个微卫星标记对于研究该物种的遗传结构和遗传多样性有效，其中15个标记适用于相关麻黄属物种。
