Goris J, Hermann J, Hendrix P, Ozon R, Merlevede W
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Belgium.
FEBS Lett. 1989 Mar 13;245(1-2):91-4. doi: 10.1016/0014-5793(89)80198-x.
Micro-injection of, or incubation with okadaic acid (OA), a specific phosphatase inhibitor, can induce formation of maturation-promoting factor (MPF) and germinal vesicle breakdown (GVBD) in Xenopus laevis oocytes. Comparison of the dose-response curves of OA on maturation, isolated enzymes and phosphatase activities in crude oocyte preparations suggests that inhibition of both polycation-stimulated (PCS) and ATP,Mg-dependent (AMD) phosphatases is sufficient but requires that a critical phosphorylation level is attained of one or several of their substrates, resulting in the formation of active MPF and meiotic maturation.
微量注射特定的磷酸酶抑制剂冈田酸（OA）或使其与非洲爪蟾卵母细胞一起孵育，可诱导成熟促进因子（MPF）的形成以及生发泡破裂（GVBD）。对OA在卵母细胞粗制品中的成熟、分离酶和磷酸酶活性方面的剂量反应曲线进行比较表明，抑制聚阳离子刺激的（PCS）和ATP、Mg依赖性的（AMD）磷酸酶就足够了，但需要其一种或几种底物达到临界磷酸化水平，从而导致活性MPF的形成和减数分裂成熟。
