Shirani Kobra, Behravan Javad, Mosaffa Fatemeh, Iranshahi Mehrdad, Mehmankhah Babak, Razavi-Azarkhiavi Kamal, Karimi Gholamreza
Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran.
Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran.
Avicenna J Phytomed. 2014 Sep;4(5):337-42.
Ferula szowitsiana has been widely used for medicinal purposes around the world. The anti-oxidant effect of F. szowitsiana had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from F. szowitsiana, against hydrogen peroxide (H2O2) - induced oxidative DNA damage in human lymphocytes.
Human lymphocytes were incubated with H2O2 (0, 25, 50, 100, and 200 µM), galbanic acid (200 and 400 µM) and a combination of galbanic acid (200 and 400 µM) and H2O2 (25 µM) at 4 C for 30 minutes. Solvents of galbanic acid without H2O2 were used as negative controls.
The findings of this study demonstrated that H2O2 exposure leads to a significant concentration-dependent increase in DNA damage. Galbanic acid did not cause DNA damage compared with the control cells. Data showed that galbanic acid does not have a protective effect against H2O2-induced oxidative DNA damage in human lymphocytes.
According to the results, it is concluded that the capability of F. szowitsiana in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients.
新疆阿魏在世界各地已被广泛用于药用。新疆阿魏的抗氧化作用已得到证实。本研究旨在确定新疆阿魏中的一种倍半萜香豆素——没药酸对过氧化氢（H2O2）诱导的人淋巴细胞氧化DNA损伤的保护作用。
将人淋巴细胞与H2O2（0、25、50、100和200 μM）、没药酸（200和400 μM）以及没药酸（200和400 μM）与H2O2（25 μM）的组合在4℃下孵育30分钟。使用不含H2O2的没药酸溶剂作为阴性对照。
本研究结果表明，暴露于H2O2会导致DNA损伤显著增加，且呈浓度依赖性。与对照细胞相比，没药酸不会导致DNA损伤。数据显示，没药酸对H2O2诱导的人淋巴细胞氧化DNA损伤没有保护作用。
根据结果得出结论，新疆阿魏在减少活性氧方面的能力及其甲醇提取物的抗炎特性可能归因于其其他成分。
