Rådinger Madeleine, Jensen Bettina M, Swindle Emily, Gilfillan Alasdair M
Krefting Research Centre, The Sahlgrenska Academy, University of Gothenburg, 424, Gothenburg, 405 30, Sweden,
Methods Mol Biol. 2015;1220:307-23. doi: 10.1007/978-1-4939-1568-2_19.
Mediator release from activated mast cells is a major initiator of the symptomology associated with allergic disorders such as anaphylaxis and asthma. Thus, methods to monitor the generation and release of such mediators have widespread applicability in studies designed to understand the processes regulating mast cell activation and for the identification of therapeutic approaches to block mast cell-driven disease. In this chapter, we discuss approaches used for the determination of mast cell degranulation, lipid-derived inflammatory mediator production, and cytokine/chemokine gene expression as well as cytokine release.
来自活化肥大细胞的介质释放是与过敏反应如过敏症和哮喘相关的症状的主要引发因素。因此，监测此类介质生成和释放的方法在旨在了解调节肥大细胞活化过程以及鉴定阻断肥大细胞驱动疾病的治疗方法的研究中具有广泛的适用性。在本章中，我们将讨论用于测定肥大细胞脱颗粒、脂质衍生的炎症介质产生、细胞因子/趋化因子基因表达以及细胞因子释放的方法。
