Fesik S W, Gampe R T, Zuiderweg E R, Kohlbrenner W E, Weigl D
NMR Research, Abbott Laboratories, Abbott Park, Illinois 60064.
Biochem Biophys Res Commun. 1989 Mar 15;159(2):842-7. doi: 10.1016/0006-291x(89)90071-5.
A heteronuclear three-dimensional NMR experiment has been applied to uniformly 15N-labeled CMP-KDO synthetase (CTP:3-deoxy-D-manno-octulosonate cytidylyl transferase; E.C. 2.7.7.38) complexed with an inhibitor and CTP. Using this 3D technique, the 2D NOE spectrum of the ternary complex was dramatically simplified by editing with respect to the 15N frequencies of the labeled enzyme. This 3D NMR method is a useful tool for resolving spectral overlap and is particularly well-suited for NMR studies of large molecules which are difficult to study by conventional 2D NMR techniques.
一种异核三维核磁共振实验已应用于与抑制剂和CTP复合的均匀15N标记的CMP-KDO合成酶（CTP：3-脱氧-D-甘露糖基辛酮酸胞苷酰转移酶；E.C. 2.7.7.38）。使用这种三维技术，通过根据标记酶的15N频率进行编辑，三元复合物的二维NOE谱被显著简化。这种三维核磁共振方法是解决光谱重叠的有用工具，特别适用于难以通过传统二维核磁共振技术研究的大分子的核磁共振研究。
