Cauda R, Laghi V, Tumbarello M, Ortona L, Whitley R J
Department of Infectious Diseases, Catholic University, Rome, Italy.
Clin Immunol Immunopathol. 1989 May;51(2):294-302. doi: 10.1016/0090-1229(89)90027-5.
Immunological studies on 12 patients with culture-proven frequently recurrent herpes simplex genitalis were performed. All the patients were evaluated at three time intervals, initially without lesions and/or within 24 hr of lesion onset (acute illness); Days 5-7 from onset and after healing (convalescence); and between recurrences (quiescence). During the first 24 hr of lesions there was a decreased number of helper (CD4+) and an increased number of suppressor/cytotoxic (CD8+) cells with a resultant decrease in the CD4/CD8 ratio. An increased proportion of CD8+ cells coexpressing the CD11 marker (suppressor cells) was noted and correlated with a low proliferative response to HSV-2 antigens. Both the NK cells (CD16+) and the NK cell activity versus HSV-2-infected targets and the K562 cell line were decreased. Five to seven days after onset the number of CD8+ cells remained increased, although the expression of CD11 marker was decreased, indicating that the majority of CD8+ cells were cytotoxic (i.e., CD8+CD11-). At this time, the lymphoproliferative response to HSV-2 antigens and NK cell activity increased, correlating both with the number of CD16+ cells and with the expression of HLA-DR on this subset. In the interval between two recurrences, no significant alteration in any of the above immunological parameters was observed.
对12例经培养证实为频繁复发性生殖器单纯疱疹的患者进行了免疫学研究。所有患者在三个时间点接受评估，最初是在无皮损时和/或皮损出现后24小时内（急性期）；发病后第5 - 7天且皮损愈合后（恢复期）；以及复发间期（静止期）。在皮损出现的最初24小时内，辅助性（CD4 +）细胞数量减少，抑制性/细胞毒性（CD8 +）细胞数量增加，导致CD4/CD8比值降低。观察到共表达CD11标志物的CD8 +细胞（抑制性细胞）比例增加，且与对HSV - 2抗原的低增殖反应相关。自然杀伤细胞（CD16 +）以及针对HSV - 2感染靶细胞和K562细胞系的自然杀伤细胞活性均降低。发病后5至7天，CD8 +细胞数量仍增加，尽管CD11标志物的表达降低，这表明大多数CD8 +细胞具有细胞毒性（即CD8 + CD11 -）。此时，对HSV - 2抗原的淋巴细胞增殖反应和自然杀伤细胞活性增加，这与CD16 +细胞数量以及该亚群上HLA - DR的表达均相关。在两次复发的间期，未观察到上述任何免疫学参数有显著改变。
