Vestey J P, Norval M, Howie S E
University Department of Dermatology, University of Edinburgh.
J R Soc Med. 1990 May;83(5):308-11. doi: 10.1177/014107689008300510.
The relationship between antibody (Ab) and lympho-proliferative responses to herpes simplex virus (HSV) and recrudescent orofacial HSV lesions were investigated in 65 patients. All had HSV-specific Ab and cell mediated immune responses (CMIR) demonstrated by ELISA and in vitro lymphoproliferation respectively. Thirteen control subjects were negative in both tests. Thirty-three patients were repeatedly investigated for 6-38 months during which time they suffered 1-8 recrudescences. HSV-induced lymphoproliferation was depressed during recrudescences, rose to a peak several weeks later, and declined slowly to a background level. However, ELISA titres and lymphoproliferative responses to Concanavalin A (Con A) were high throughout and circulating peripheral blood mononuclear cell (PBMC) subsets did not change. Depressed lymphoproliferative responses to HSV antigen (Ag) during recrudescences were enhanced by removal of CD8+ cells from PBMC using either a panning technique or cell sorting; reconstitution of CD8+ cells suppressed the HSV-specific lymphoproliferative response. CD8+ cell depletion affected neither HSV-induced lymphoproliferation recrudescence, nor lymphoproliferative responses to another Ag (PPD) during recrudescence. Depressed HSV-induced lymphoproliferation during recrudescences might thus be due to CD8+ suppressor T cell (Ts) function rather than low numbers of circulating lymphocytes. Suppression or delay of normal CMIR to asymptomatic recurrent epidermal HSV infection by Ts might allow development of recrudescent HSV lesions.
在65例患者中研究了抗体（Ab）与单纯疱疹病毒（HSV）淋巴细胞增殖反应以及复发性口腔面部HSV损伤之间的关系。所有患者均分别通过ELISA和体外淋巴细胞增殖试验证明具有HSV特异性抗体和细胞介导的免疫反应（CMIR）。13名对照受试者在两项试验中均为阴性。33例患者被反复研究6 - 38个月，在此期间他们经历了1 - 8次复发。HSV诱导的淋巴细胞增殖在复发期间受到抑制，数周后升至峰值，然后缓慢下降至背景水平。然而，ELISA滴度和对刀豆球蛋白A（Con A）的淋巴细胞增殖反应在整个过程中都很高，并且循环外周血单核细胞（PBMC）亚群没有变化。在复发期间，通过使用淘选技术或细胞分选从PBMC中去除CD8 +细胞可增强对HSV抗原（Ag）的淋巴细胞增殖反应降低；重新加入CD8 +细胞可抑制HSV特异性淋巴细胞增殖反应。CD8 +细胞耗竭既不影响HSV诱导的淋巴细胞增殖复发，也不影响复发期间对另一种抗原（PPD）的淋巴细胞增殖反应。因此，复发期间HSV诱导的淋巴细胞增殖降低可能是由于CD8 +抑制性T细胞（Ts）功能，而不是循环淋巴细胞数量减少。Ts对无症状复发性表皮HSV感染的正常CMIR的抑制或延迟可能会导致复发性HSV损伤的发生。
