Man Y G, Chen M H
Department of Cell Biology, Virus Research Institute, Hubei Medical College, Wuhan, China.
Gynecol Oncol. 1989 Apr;33(1):27-33. doi: 10.1016/0090-8258(89)90597-0.
Hybrid Swiss virgin mice (Kuan-min strain) were challenged intravaginally with uv-inactivated herpes simplex virus type 1 (HSV-1) or type 2 (HSV-2) twice a week for 16 times and subsequently with croton oil or control medium twice a week for 27 times. After a period of 180 days the exposure to HSV-2 plus control medium induced premalignant and malignant lesions of the cervix and vagina in 50.0% of the mice. The exposure to HSV-2 plus croton oil induced similar lesions in 78.2% of the mice. The exposure to HSV-1 combined with either control medium or croton oil induced lesions in 37.2 and 58.3% of the mice, respectively. No malignant lesions were found in the control mice. These results reveal that (1) HSV-1 and HSV-2 have a similar oncogenic potential, and (2) croton oil can promote the induction of cervical and vaginal cancers with HSV-1 and HSV-2. Additionally, these results suggest that the virus-induced carcinogenesis of cervix and vagina might be similar to the classical two-stage carcinogenesis model. This animal model seems to be more suitable for studying the prevention and treatment of cervical and vaginal cancers because the period of this experiment is significantly shorter than that of experiments reported by other investigators and the frequency of the malignant lesion is significantly higher than or similar to that of the same lesion reported in other experiments.
将杂种瑞士处女小鼠（宽敏品系）每周经阴道用紫外线灭活的单纯疱疹病毒1型（HSV-1）或2型（HSV-2）攻击两次，共16次，随后每周用巴豆油或对照培养基处理两次，共27次。180天后，暴露于HSV-2加对照培养基的小鼠中有50.0%发生了子宫颈和阴道的癌前病变和恶性病变。暴露于HSV-2加巴豆油的小鼠中有78.2%发生了类似病变。暴露于HSV-1分别与对照培养基或巴豆油联合处理的小鼠中，病变发生率分别为37.2%和58.3%。对照小鼠未发现恶性病变。这些结果表明：（1）HSV-1和HSV-2具有相似的致癌潜能；（2）巴豆油可促进HSV-1和HSV-2诱导子宫颈和阴道癌。此外，这些结果提示病毒诱导的子宫颈和阴道癌变可能类似于经典的两阶段致癌模型。该动物模型似乎更适合于研究子宫颈和阴道癌的防治，因为本实验周期明显短于其他研究者报道的实验，且恶性病变发生率明显高于或类似于其他实验报道的相同病变发生率。
