Jancar S, Braquet P, Sirois P
Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Int J Immunopharmacol. 1989;11(2):129-32. doi: 10.1016/0192-0561(89)90064-7.
An Arthus reaction was induced in the rat peritoneal cavity. The inflammatory exudates were collected 10 min after induction of the reaction and analysed for the presence of prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) by enzyme immunoassays (EIA), and of leukotriene B4 (LTB4) by radioimmunoassay. Our results showed that control release (CONT) of eicosanoids in the peritoneal cavity averaged 2.3 ng/ml for TXB2, 0.21 ng/ml for PGE2 and 18 pg/ml for LTB4. Following antigen challenge, the levels of TXB2, PGE2 and LTB4 in the peritoneal cavity increased to 17.0 ng/ml, 0.41 ng/ml and 49.0 pg/ml, respectively. Indomethacin totally inhibited the release of PGE2 and TXB2 whereas it increased by 326% the release of LTB4. The PAF antagonist, BN-52021 significantly inhibited (around 40%) the release of LTB4 in rat peritoneal cavity, increased the release of PGE2, and did not affect the release of TXB2. These results clearly suggest a mediatory role for both cyclooxygenase and lipoxygenase products in Arthus reaction and provide evidence that PAF is also involved in complex interactions with the eicosanoids.
在大鼠腹腔诱导产生阿瑟斯反应。在反应诱导后10分钟收集炎性渗出物，通过酶免疫测定法（EIA）分析前列腺素E2（PGE2）和血栓素B2（TXB2）的存在情况，通过放射免疫测定法分析白三烯B4（LTB4）的存在情况。我们的结果显示，腹腔中类花生酸的对照释放量（CONT）平均为TXB2 2.3 ng/ml、PGE2 0.21 ng/ml和LTB4 18 pg/ml。抗原攻击后，腹腔中TXB2、PGE2和LTB4的水平分别增至17.0 ng/ml、0.41 ng/ml和49.0 pg/ml。吲哚美辛完全抑制PGE2和TXB2的释放，而它使LTB4的释放增加了326%。血小板活化因子拮抗剂BN - 52021显著抑制（约40%）大鼠腹腔中LTB4的释放，增加PGE2的释放，并且不影响TXB2的释放。这些结果清楚地表明环氧化酶和脂氧化酶产物在阿瑟斯反应中起介导作用，并提供证据表明血小板活化因子也参与与类花生酸的复杂相互作用。
