Houwers D J, Nauta I M
Central Veterinary Institute, Lelystad, The Netherlands.
Vet Microbiol. 1989 Feb;19(2):127-39. doi: 10.1016/0378-1135(89)90078-3.
The antibody response of sheep to maedi-visna virus (MVV) infection was studied, using an immunoblotting technique that identified the four major viral structural proteins: the envelope glycoprotein gp135, and internal proteins p25, p16 and p14. In sequential serum samples of two inoculated sheep, antibodies to p25 appeared first, soon followed by antibodies to p16. In two sheep with natural infections, which were sampled with longer intervals, antibodies to p25 and p16 appeared simultaneously. Antibody response to p14, which was weak and inconsistent in most cases, appeared after the p16 response. Antibodies to gp135 were detected in only one sheep and appeared 7 weeks after those to p25. Antibodies to p25 were particularly persistent. Antibody recognition patterns of 21 necropsied sheep with naturally occurring infections were compared. Sheep with lesions lacked antibodies to p14 and p16 and sometimes even p25, although these antibodies had been present in earlier stages of infection. Since the time of onset of lesions was unknown, we could not determine whether this decline in antibody activity occurred before, together with, or after the onset of lesion development.
利用免疫印迹技术研究了绵羊对梅迪 - 维斯纳病毒（MVV）感染的抗体反应，该技术可识别四种主要病毒结构蛋白：包膜糖蛋白gp135以及内部蛋白p25、p16和p14。在两只接种绵羊的连续血清样本中，针对p25的抗体首先出现，随后很快出现针对p16的抗体。在两只自然感染的绵羊中，采样间隔较长，针对p25和p16的抗体同时出现。针对p14的抗体反应在大多数情况下较弱且不一致，在针对p16的反应之后出现。仅在一只绵羊中检测到针对gp135的抗体，且在针对p25的抗体出现7周后出现。针对p25的抗体尤其持久。比较了21只自然感染剖检绵羊的抗体识别模式。有病变的绵羊缺乏针对p14和p16的抗体，有时甚至缺乏针对p25的抗体，尽管这些抗体在感染早期曾出现过。由于病变开始的时间未知，我们无法确定抗体活性的这种下降是在病变发展开始之前、同时还是之后发生的。
