评估两种重组梅迪-维斯纳病毒蛋白在用于检测绵羊慢病毒血清抗体的酶联免疫吸附测定中的应用。
Evaluation of two recombinant Maedi-visna virus proteins for use in an enzyme-linked immunosorbent assay for the detection of serum antibodies to ovine lentiviruses.
作者信息
Power C, Richardson S, Briscoe M, Pasick J
机构信息
Health of Animals Laboratory, Agriculture Canada, Sackville, New Brunswick, Canada.
出版信息
Clin Diagn Lab Immunol. 1995 Sep;2(5):631-3. doi: 10.1128/cdli.2.5.631-633.1995.
Defined segments of the gag polyprotein and transmembrane envelope glycoprotein from Maedi-visna virus were expressed as glutathione S-transferase fusion proteins in Escherichia coli and evaluated singly and in combination for use in an enzyme-linked immunosorbent assay (ELISA). Two hundred sixty field serum specimens from 15 sheep flocks were tested in parallel with recombinant and whole-virus antigens, and the relative sensitivities and specificities of the recombinant antigens were calculated. When the recombinant gag and transmembrane proteins were used in combination, a sensitivity of 97.4% and a specificity of 99.4% relative to whole-virus antigen were observed, indicating the utility of these proteins in diagnostic testing.
将梅迪-维斯纳病毒gag多聚蛋白和跨膜包膜糖蛋白的特定片段作为谷胱甘肽S-转移酶融合蛋白在大肠杆菌中表达，并单独和联合评估其在酶联免疫吸附测定（ELISA）中的应用。对来自15个羊群的260份现场血清样本与重组抗原和全病毒抗原进行平行检测，并计算重组抗原的相对敏感性和特异性。当联合使用重组gag蛋白和跨膜蛋白时，相对于全病毒抗原观察到的敏感性为97.4%，特异性为99.4%，表明这些蛋白在诊断检测中的实用性。
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