Raska I, Reimer G, Jarník M, Kostrouch Z, Raska K
Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague.
Biol Cell. 1989;65(1):79-82.
By means of immunocytochemistry performed on cryosections of cultured cells, RNA polymerase I was localized mainly to nucleolar fibrillar centers. The labelling of nucleolar dense fibrillar components was low and depended on the cell type. In contrast, DNA topoisomerase I and RNP complexes containing U3 snRNA were enriched in dense fibrillar components, their occurrence in fibrillar centers being usually much less.
通过对培养细胞冰冻切片进行免疫细胞化学分析，发现RNA聚合酶I主要定位于核仁纤维中心。核仁致密纤维成分的标记较弱，且取决于细胞类型。相比之下，DNA拓扑异构酶I和含有U3小核仁RNA的核糖核蛋白复合体在致密纤维成分中富集，它们在纤维中心的出现通常要少得多。
