Lou Guangyuan, Zhang Yongjun, Bao Wenlong, Deng Dehou
Department of Chemotherapy, Zhejiang Cancer Hospital, Hangzhou, China.
Department of Integration of Traditional Chinese and Western Medicine, Zhejiang Cancer Hospital, Hangzhou, China.
Acta Biochim Pol. 2014;61(4):765-8. Epub 2014 Nov 14.
Aims. This study aimed to investigate CHRNA3 (rs8040868) and PHACTR2 (rs9390123) single-nucleotide polymorphisms (SNPs) for association with non-small-cell lung cancer (NSCLC) risk in a Chinese population, and whether the environment affects the genetic polymorphisms. Methods. This case and control study included 500 NSCLC patients and 500 age-matched healthy controls. CHRNA3 (rs8040868) and PHACTR2 (rs9390123) SNPs were genotyped and associated for NSCLC risk by computing the odds ratio and 95% confidence interval from multivariate unconditional logistic regression analyses with adjustment of age. Results. The minor allele frequency (MAF) of CHRNA3 (rs8040868) and PHACTR2 (rs9390123) was 0.350 (C) and 0.397 (C), respectively. The frequencies of genotype and allele in CHRNA3 (rs8040868) and PHACTR2 (rs9390123) were not significantly different between the cases and controls, or between either of the subgroups. Conclusion. Although rs8040868 and rs9390123 SNPs are not associated with NSCLC risk in Chinese population, the results strongly suggest that geographical agents interact with human genetic polymorphism independent of ethnic background.
目的。本研究旨在调查中国人群中CHRNA3（rs8040868）和PHACTR2（rs9390123）单核苷酸多态性（SNP）与非小细胞肺癌（NSCLC）风险的相关性，以及环境是否影响基因多态性。方法。这项病例对照研究纳入了500例NSCLC患者和500例年龄匹配的健康对照。通过计算多因素无条件逻辑回归分析调整年龄后的比值比和95%置信区间，对CHRNA3（rs8040868）和PHACTR2（rs9390123）SNP进行基因分型并与NSCLC风险相关联。结果。CHRNA3（rs8040868）和PHACTR2（rs9390123）的次要等位基因频率（MAF）分别为0.350（C）和0.397（C）。病例组和对照组之间，或任何一个亚组之间，CHRNA3（rs8040868）和PHACTR2（rs9390123）的基因型和等位基因频率均无显著差异。结论。尽管rs8040868和rs9390123 SNP与中国人群的NSCLC风险无关，但结果强烈表明地理因素与人类基因多态性相互作用，且独立于种族背景。
