伊朗男性中PD-1基因多态性与抗精子抗体相关不育症之间的关联。
Association between a PD-1 gene polymorphism and antisperm antibody-related infertility in Iranian men.
作者信息
Zamani Mohammad Reza, Asbagh Firouzeh Akbari, Massoud Amir Hossein, Salmaninejad Arash, Massoud Ahmad, Rezaei Nima
机构信息
Department of Immunology and Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran,
出版信息
J Assist Reprod Genet. 2015 Jan;32(1):103-6. doi: 10.1007/s10815-014-0371-4. Epub 2014 Nov 16.
OBJECTIVE
Programmed cell death-1 (PD-1, Pdcd1), an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signalling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. This study investigates PD-1 gene polymorphism in patients with antisperm antibody-related infertility
METHODS
Genotyping was performed by polymerase chain reaction and restriction enzyme digestion (PCR-RFLP), this polymorphism was genotyped in 145 Iranian subjects (61 patients with antisperm antibody-related infertility and 84 healthy controls).
RESULTS
Patients frequencies of the G/A genotype in comparison with healthy controls (38.2 % vs. 32.7 %, OR =1.21, P = 0.35) were not significantly different. However, G/G and A/A genotype frequencies between patients and healthy controls were significantly different (P = 0.042, P = 0.00001, respectively). Also, allele frequencies of this polymorphism were significantly different (P = 0.0012) in patients compared to healthy controls.
CONCLUSION
According to these results, there is a correlation between PD-1 gene polymorphism and susceptibility to antisperm antibody-related infertility in our study group.
目的
程序性细胞死亡蛋白1（PD-1，Pdcd1）是一种属于CD28/CTLA-4家族的免疫受体，在与两种配体（PD-L1或PD-L2）之一相互作用时，通过招募蛋白酪氨酸磷酸酶SHP-2来负向调节抗原受体信号传导。本研究调查抗精子抗体相关不孕症患者的PD-1基因多态性。
方法
采用聚合酶链反应和限制性内切酶消化法（PCR-RFLP）进行基因分型，对145名伊朗受试者（61例抗精子抗体相关不孕症患者和84名健康对照）进行该多态性基因分型。
结果
患者的G/A基因型频率与健康对照相比（38.2%对32.7%，OR =1.21，P = 0.35）无显著差异。然而，患者与健康对照之间的G/G和A/A基因型频率有显著差异（分别为P = 0.042，P = 0.00001）。此外，该多态性的等位基因频率在患者与健康对照之间也有显著差异（P = 0.0012）。
结论
根据这些结果，在我们的研究组中，PD-1基因多态性与抗精子抗体相关不孕症的易感性之间存在相关性。
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