Grant Irene R, Stewart Linda D
Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast,, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK,
Methods Mol Biol. 2015;1247:153-61. doi: 10.1007/978-1-4939-2004-4_11.
Immunomagnetic separation (IMS) represents a simple but effective method of selectively capturing and concentrating Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with M. bovis-specific antibody and peptide binders. Once captured by IMS, M. bovis cells can be detected by either PCR or cultural detection methods. Increased detection rates of M. bovis, particularly from non-visibly lesioned lymph node tissues from bTB reactor animals, have recently been reported when IMS-based methods were employed.
免疫磁珠分离（IMS）是一种从组织样本中选择性捕获和浓缩牛分枝杆菌（牛结核病（bTB）的病原体）的简单而有效的方法。它是一种物理细胞分离技术，与培养前的传统化学去污方法不同，不会影响细胞活力。IMS使用包被有牛分枝杆菌特异性抗体和肽结合剂的顺磁性珠子进行。一旦被IMS捕获，牛分枝杆菌细胞可以通过PCR或培养检测方法进行检测。最近有报道称，当采用基于IMS的方法时，牛分枝杆菌的检测率有所提高，特别是从bTB反应动物的非可见病变淋巴结组织中检测到的。
