Bhattacherjee Amrita, Mallik Saurav, Kundu Sudip
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata, 700009, India.
J Mol Evol. 2015 Jan;80(1):10-2. doi: 10.1007/s00239-014-9654-8. Epub 2014 Nov 16.
A compensatory mutation (CM) counter balances lethal effects of a deleterious mutation (DM), ensuring the persistence of both through natural selection. However, little is known about the biological aspects of CMs those restore the structural alterations of proteins caused by slightly DMs. Here, by analyzing the evolution of the UDP-glycosyltransferase 73B4 protein among monocot-dicot plants, we investigate the occurrence of CMs around slightly DMs in 3D space. Our results illustrate that CMs exhibit significantly higher tendency to occur within the range of electrostatic interaction around the slightly DMs, compared to occurring randomly in the protein.
补偿性突变（CM）可抵消有害突变（DM）的致死效应，通过自然选择确保两者都能留存下来。然而，对于那些能恢复由轻度有害突变引起的蛋白质结构改变的补偿性突变的生物学特性，我们却知之甚少。在此，通过分析单子叶植物和双子叶植物中UDP-糖基转移酶73B4蛋白的进化情况，我们研究了三维空间中轻度有害突变周围补偿性突变的发生情况。我们的结果表明，与在蛋白质中随机发生相比，补偿性突变在轻度有害突变周围的静电相互作用范围内发生的倾向要高得多。
