BLAST：一系列强大且多样的序列分析工具的核心。
BLAST: at the core of a powerful and diverse set of sequence analysis tools.
作者信息
McGinnis Scott, Madden Thomas L
机构信息
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 38A, 8600 Rockville Pike, Bethesda, MD 20894, USA.
出版信息
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W20-5. doi: 10.1093/nar/gkh435.
Abstract
Basic Local Alignment Search Tool (BLAST) is one of the most heavily used sequence analysis tools available in the public domain. There is now a wide choice of BLAST algorithms that can be used to search many different sequence databases via the BLAST web pages (http://www.ncbi.nlm.nih.gov/BLAST/). All the algorithm-database combinations can be executed with default parameters or with customized settings, and the results can be viewed in a variety of ways. A new online resource, the BLAST Program Selection Guide, has been created to assist in the definition of search strategies. This article discusses optimal search strategies and highlights some BLAST features that can make your searches more powerful.
摘要
基本局部比对搜索工具（BLAST）是公共领域中使用最为频繁的序列分析工具之一。目前有多种BLAST算法可供选择，可通过BLAST网页（http://www.ncbi.nlm.nih.gov/BLAST/）搜索许多不同的序列数据库。所有算法与数据库的组合都可以使用默认参数或自定义设置来执行，结果也可以通过多种方式查看。现已创建了一个新的在线资源——BLAST程序选择指南，以协助定义搜索策略。本文讨论了最佳搜索策略，并重点介绍了一些可使搜索更强大的BLAST功能。
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