预孵育后猪脂肪组织的脂解活性

Lipolytic activity of porcine adipose tissue after preincubation.

Mersmann H J

U.S. Department of Agriculture, Clay Center, NE 68933.

Comp Biochem Physiol B. 1989;92(3):493-9. doi: 10.1016/0305-0491(89)90122-3.

Abstract

The stimulated lipolytic rate in porcine adipose tissue slices was markedly increased after preincubation with or without isoproterenol. 2. Preincubation with and without insulin, dibutyryl-cAMP or theophylline suggested that the activation of the stimulated rate resulted from inhibition or inactivation of cAMP-phosphodiesterase activity during preincubation. 3. Preincubation with isoproterenol also caused activation of the basal (no exogenous hormone) lipolytic activity. 4. However, much of this activation appeared to result from carry-over of isoproterenol into the incubation medium because it could be lowered by additional washing of the tissue, by lower isoproterenol concentration or by propranolol.

摘要

用或不用异丙肾上腺素预孵育后，猪脂肪组织切片中刺激的脂解速率显著增加。2. 用或不用胰岛素、二丁酰环磷腺苷或茶碱预孵育表明，预孵育期间环磷腺苷磷酸二酯酶活性的抑制或失活导致了刺激速率的激活。3. 用异丙肾上腺素预孵育也会导致基础（无外源激素）脂解活性的激活。4. 然而，这种激活似乎很大程度上是由于异丙肾上腺素残留到孵育培养基中，因为通过对组织进行额外洗涤、降低异丙肾上腺素浓度或使用普萘洛尔可以降低这种激活。