Burns T W, Terry B E, Langley P E, Robison G A
Diabetes. 1979 Nov;28(11):957-61. doi: 10.2337/diab.28.11.957.
To gain information on the manner in which insulin suppresses lipolysis in man, isolated adipocytes, prepared from subcutaneous adipose tissue, were incubated with insulin (100 microunits/ml) alone and in combination with isoproterenol (10(-7) M or 10(-8) M). Cyclic AMP concentration was measured at 60 min; glycerol release, used as an index of lipolysis, was determined at 45 and 75 min. Insulin consistently reduced both basal and stimulated cyclic AMP and glycerol release: the degree of suppression of each was comparable. In subsequent experiments, the ability of insulin to suppress glycerol release stimulated by isoproterenol, theophylline, and dibutyryl cyclic AMP (dbcAMP), respectively, was compared. Insulin substantially reduced the raised levels of cyclic AMP and glycerol release prompted by isoproterenol and theophylline, but it had little effect on increases caused by dbcAMP. These findings support the view that reduction in cyclic AMP is an important component in the regulation of fat mobilization by insulin.
为了获取胰岛素抑制人体脂肪分解方式的相关信息，从皮下脂肪组织制备的分离脂肪细胞分别与单独的胰岛素（100微单位/毫升）以及与异丙肾上腺素（10⁻⁷M或10⁻⁸M）联合孵育。在60分钟时测量环磷酸腺苷（cAMP）浓度；在45分钟和75分钟时测定甘油释放量，将其作为脂肪分解的指标。胰岛素持续降低基础和刺激状态下的cAMP以及甘油释放量：对每种的抑制程度相当。在后续实验中，比较了胰岛素分别抑制异丙肾上腺素、茶碱和二丁酰环磷腺苷（dbcAMP）刺激的甘油释放的能力。胰岛素显著降低了异丙肾上腺素和茶碱促使的升高的cAMP水平和甘油释放量，但对dbcAMP引起的增加几乎没有影响。这些发现支持了这样一种观点，即cAMP的降低是胰岛素调节脂肪动员的一个重要组成部分。
