甲巯咪唑对促甲状腺激素和胰岛素样生长因子I诱导的大鼠甲状腺细胞生长的直接作用。

Direct effect of methimazole on rat thyroidal cell growth induced by thyrotropin and insulin-like growth factor I.

作者信息

Taniguchi S, Yoshida A, Mashiba H

机构信息

First Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.

出版信息

Endocrinology. 1989 May;124(5):2046-51. doi: 10.1210/endo-124-5-2046.

DOI:10.1210/endo-124-5-2046

PMID:2539965

Abstract

The direct effect of methimazole (MMI) on FRTL-5 cell growth was examined. TSH, (Bu)2cAMP, calf serum, insulin-like growth factor I, and Graves' immunoglobulin (IgG) increased [3H]thymidine incorporation into DNA during 72-h incubation. MMI (10(-3) M), which does not damage cell viability, significantly enhanced the increase in [3H]thymidine incorporation induced by TSH and (Bu)2cAMP. In contrast, MMI suppressed the increase in [3H]thymidine incorporation induced by calf serum, insulin-like growth factor I, and Graves' IgG. MMI had no effect on the production of cAMP by TSH. Accordingly, we concluded that MMI has opposite effects on cAMP- and non-cAMP-dependent cell growth pathways. Moreover, Graves' IgG, which has a modest effect on cAMP production, is believed to induce cell growth via the non-cAMP dependent cell growth pathway.

摘要

研究了甲巯咪唑（MMI）对FRTL-5细胞生长的直接影响。在72小时的孵育过程中，促甲状腺激素（TSH）、双丁酰环磷腺苷（Bu）2cAMP、小牛血清、胰岛素样生长因子I和格雷夫斯病免疫球蛋白（IgG）均增加了[3H]胸腺嘧啶核苷掺入DNA的量。不损害细胞活力的MMI（10（-3）M）显著增强了TSH和（Bu）2cAMP诱导的[3H]胸腺嘧啶核苷掺入量的增加。相反，MMI抑制了小牛血清、胰岛素样生长因子I和格雷夫斯病IgG诱导的[3H]胸腺嘧啶核苷掺入量的增加。MMI对TSH产生环磷腺苷（cAMP）没有影响。因此，我们得出结论，MMI对cAMP依赖性和非cAMP依赖性细胞生长途径具有相反的作用。此外，对cAMP产生有适度影响的格雷夫斯病IgG被认为通过非cAMP依赖性细胞生长途径诱导细胞生长。