B7-H4表达与尿路上皮细胞癌的肿瘤进展及临床结局相关。
B7-H4 expression is correlated with tumor progression and clinical outcome in urothelial cell carcinoma.
作者信息
Fan Min, Zhuang Qianfeng, Chen Yiming, Ding Tao, Yao Hongwei, Chen Lujun, He Xiaozhou, Xu Xianlin
机构信息
Department of Urology, The Third Affiliated Hospital of Soochow University Changzhou 213000, Jiangsu, China.
Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University Changzhou 213000, Jiangsu, China.
出版信息
Int J Clin Exp Pathol. 2014 Sep 15;7(10):6768-75. eCollection 2014.
OBJECTIVE
To investigate the mRNA and protein levels of B7-H4, a B7 family molecule, in human urothelial cell carcinoma (UCC), to analyze the relationship between B7-H4 protein expression level and pathological stage of UCC, and to examine the potential of B7-H4 as a prognostic factor in UCC.
METHODS
mRNA and protein levels of B7-H4 were measured in pairs of tumor tissues and matched adjacent nontumor tissue obtained from patients with UCC by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemical staining, respectively. Association of the protein level of B7-H4 with pathological tumor stage and the overall survival of UCC patients were also analyzed.
RESULTS
B7-H4 mRNA and protein level were significantly higher in UCC tumor tissues compared with adjacent nontumor tissues as assessed by qRT-PCR and immunohistochemical staining, respectively. Higher B7-H4 protein levels were observed in patients with more advanced pathological stage of UCC and were also associated with decreased overall survival of patients with UCC.
CONCLUSIONS
The findings from this study indicate that B7-H4 has the potential to be an independent prognostic indicator for UCC.
目的
研究B7家族分子B7-H4在人膀胱尿路上皮癌（UCC）中的mRNA和蛋白水平，分析B7-H4蛋白表达水平与UCC病理分期的关系，并探讨B7-H4作为UCC预后因素的可能性。
方法
分别采用定量逆转录-聚合酶链反应（qRT-PCR）和免疫组织化学染色法，检测UCC患者肿瘤组织及其配对的癌旁非肿瘤组织中B7-H4的mRNA和蛋白水平。分析B7-H4蛋白水平与UCC患者病理肿瘤分期及总生存的相关性。
结果
通过qRT-PCR和免疫组织化学染色评估，UCC肿瘤组织中B7-H4的mRNA和蛋白水平分别显著高于癌旁非肿瘤组织。UCC病理分期越晚的患者，B7-H4蛋白水平越高，且其也与UCC患者总生存降低相关。
结论
本研究结果表明，B7-H4有可能成为UCC的独立预后指标。
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