An Huai-Jie, Zhou Chang-Xi, Geng Peiliang, Xu Hong-Tao, Shi Chenghe, Zhao Xiao-Hang, Qian Yang-Ming
Center of Basic Medical Sciences, Navy General Hospital of PLA Beijing 100037, P. R. China.
Department of Nanlou Respiratory Disease, Chinese PLA General Hospital Beijing 100853, P. R. China.
Int J Clin Exp Pathol. 2014 Sep 15;7(10):6980-4. eCollection 2014.
The purpose of this study was to explore the effect of PERIOD3 (PER3) genotypes on circadian rhythmicity in flight cadets after militarized management.
We performed a preliminary study in 146 newly enrolled male flight cadets. Venous blood samples were collected, and genotyping of PER3 (4/5) was determined by using PCR. The morningness-eveningness questionnaire (MEQ) survey was given to flight cadets upon enrollment and after militarized management for 24 months respectively. Comparison of frequency distribution of PER3 genotypes between cases and controls (120 well-matched civilians) was performed using the X(2) test. We also compared the circadian rhythmicity upon enrollment and 24 months after enrollment in flight cadets, and analyzed the connection of changes in circadian clock with PER3 genotypes.
The frequency distribution of PER3 genotypes in flight cadets was not significantly different from that in controls subjects. MEQ survey results showed chronotype within flight cadet group varied widely at the two time-points: the moderately morning type (50%) and the neither type (41.1%) upon enrollment; the neither type (76.7%) and the moderately morning type (21.2%) 24 months after enrollment. The circadian rhythm of individuals with the PER3 (5/5) genotype showed no significant difference before and after 24 months of militarized management, whereas notable changes were found in individuals with the PER3 (4/4) genotype (n=116, X(2) =37.26, P < 0.001).
In conclusion, we provide some evidence that circadian rhythm of flight cadets with the PER3 (5) allele are less likely to be affected compared to those with the PER3 (4) allele.
本研究旨在探讨PERIOD3（PER3）基因多态性对军事管理后飞行学员昼夜节律的影响。
对146名新入学男性飞行学员进行初步研究。采集静脉血样本，采用聚合酶链反应（PCR）法检测PER3（4/5）基因分型。分别在入学时和军事管理24个月后对飞行学员进行晨型-夜型问卷（MEQ）调查。采用X²检验比较病例组（飞行学员）和对照组（120名匹配良好的平民）中PER3基因多态性的频率分布。我们还比较了飞行学员入学时和入学24个月后的昼夜节律，并分析了生物钟变化与PER3基因多态性的关系。
飞行学员中PER3基因多态性的频率分布与对照组无显著差异。MEQ调查结果显示，飞行学员组在两个时间点的昼夜类型差异较大：入学时中度晨型（50%）和中间型（41.1%）；入学24个月后中间型（76.7%）和中度晨型（21.2%）。PER3（5/5）基因型个体在军事管理24个月前后的昼夜节律无显著差异，而PER3（4/4）基因型个体（n = 116，X² = 37.26，P < 0.001）有显著变化。
总之，我们提供了一些证据表明，与携带PER3（4）等位基因的飞行学员相比，携带PER3（5）等位基因的飞行学员的昼夜节律受影响的可能性较小。
