Gong Wenfang, He Shoupu, Tian Jiahuan, Sun Junling, Pan Zhaoe, Jia Yinhua, Sun Gaofei, Du Xiongming
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China; Department of Computer Science and Information Engineering, Anyang Institute of Technology, Anyang, China.
PLoS One. 2014 Nov 17;9(11):e112966. doi: 10.1371/journal.pone.0112966. eCollection 2014.
To understand the mechanism of fiber development and pigmentation formation, the mRNAs of two cotton lines were sequenced: line Z128 (light brown fiber) was a selected mutant from line Z263 (dark brown fiber). The primary walls of the fiber cell in both Z263 and Z128 contain pigments; more pigments were laid in the lumen of the fiber cell in Z263 compared with that in Z128. However, Z263 contained less cellulose than Z128. A total of 71,895 unigenes were generated: 13,278 (20.26%) unigenes were defined as differentially expressed genes (DEGs) by comparing the library of Z128 with that of Z263; 5,345 (8.16%) unigenes were up-regulated and 7,933 (12.10%) unigenes were down-regulated. qRT-PCR and comparative transcriptional analysis demonstrated that the pigmentation formation in brown cotton fiber was possibly the consequence of an interaction between oxidized tannins and glycosylated anthocyanins. Furthermore, our results showed the pigmentation related genes not only regulated the fiber color but also influenced the fiber quality at the fiber elongation stage (10 DPA). The highly expressed flavonoid gene in the fiber elongation stage could be related to the fiber quality. DEGs analyses also revealed that transcript levels of some fiber development genes (Ca(2+)/CaM, reactive oxygen, ethylene and sucrose phosphate synthase) varied dramatically between these two cotton lines.
为了解纤维发育和色素沉着形成的机制，对两个棉花品系的mRNA进行了测序：Z128品系（浅棕色纤维）是从Z263品系（深棕色纤维）中选育出的突变体。Z263和Z128纤维细胞的初生壁均含有色素；与Z128相比，Z263纤维细胞腔中沉积的色素更多。然而，Z263的纤维素含量低于Z128。共产生了71,895个单基因：通过比较Z128和Z263的文库，将13,278个（20.26%）单基因定义为差异表达基因（DEG）；5,345个（8.16%）单基因上调，7,933个（12.10%）单基因下调。qRT-PCR和比较转录分析表明，棕色棉纤维中的色素沉着形成可能是氧化单宁和糖基化花青素相互作用的结果。此外，我们的结果表明，色素沉着相关基因不仅调控纤维颜色，还在纤维伸长阶段（开花后10天）影响纤维品质。纤维伸长阶段高表达的类黄酮基因可能与纤维品质有关。DEG分析还显示，这两个棉花品系之间一些纤维发育基因（Ca(2+)/CaM、活性氧、乙烯和蔗糖磷酸合酶）的转录水平差异显著。
