Brodin L, Ohta Y, Hökfelt T, Grillner S
Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.
J Comp Neurol. 1989 Mar 8;281(2):225-33. doi: 10.1002/cne.902810206.
The distribution of radiolabeled neurons in the brain stem of Lampetra fluviatilis was studied following unilateral injections of (3H)D-aspartate in the rostral spinal cord. After survival periods of 1-3 days, labeled perikarya were present within and nearby the posterior, middle, and anterior rhombencephalic reticular nuclei and in the mesencephalic reticular nucleus. The highest number of (3H)D-aspartate labeled cell bodies were present in the posterior rhombencephalic reticular nucleus. The labeled reticulospinal neurons were distributed mainly ipsilateral to the injection site and included the giant Müller cells as well as medium-sized and small neurons. Contralateral labeling occurred in cell bodies scattered along the lateral margin of the rhombencephalic reticular formation, the most rostral of these contralaterally projecting neurons being the Mauthner cell. The (3H)D-aspartate labeling correlates with previous electrophysiological studies showing that lamprey reticulospinal neurons utilize excitatory amino acid transmission.
在七鳃鳗的吻部脊髓单侧注射（3H）D-天冬氨酸后，研究了放射性标记神经元在其脑干中的分布。在1 - 3天的存活期后，标记的神经细胞体出现在后脑后部、中部和前部网状核内及其附近，以及中脑网状核中。后脑后部网状核中存在的（3H）D-天冬氨酸标记细胞体数量最多。标记的网状脊髓神经元主要分布在注射部位的同侧，包括巨大的米勒细胞以及中型和小型神经元。对侧标记出现在沿后脑网状结构外侧边缘散布的细胞体中，这些对侧投射神经元中最靠前的是莫特纳尔细胞。（3H）D-天冬氨酸标记与先前的电生理研究相关，该研究表明七鳃鳗网状脊髓神经元利用兴奋性氨基酸传递。
