Ilbäck N G, Fohlman J, Slorach S, Friman G
Toxicology Laboratory, Swedish National Food Administration, Uppsala.
J Immunol. 1989 May 1;142(9):3225-8.
Quinoline-3-carboxamide (LS 2616) is a broadly acting immunostimulator with anti-inflammatory effects in Coxsackie virus B3-induced myocarditis in female BALB/c mice. This infection caused extensive inflammatory and necrotic lesions in the myocardium 7 days after inoculation (6.8% of tissue section area). The damaged area was reduced (to 3.7% (p less than 0.05] and the lethality decreased when LS 2616 was administered over 14 days, starting 7 days before the inoculation. The response pattern of lymphocyte subsets in situ in myocardial inflammatory lesions was elucidated by a newly developed immune histochemical staining technique. LS 2616 increased the number of class II-expressing cells 3-fold (p less than 0.01) and the CTL, Ts:Th cell ratio by 55% (p less than 0.05), whereas Lyt-1+ and TIB+ cells were unaffected. After 7 days of LS 2616 treatment, spleen lymphocyte activity tended to increase (T cells by 21% (NS) and B cells by 60% (p less than 0.05), respectively). The activity of NK cells increased by 51% (p less than 0.01). LS 2616 may thus have potential in therapy of human inflammatory disorders, such as myocarditis.
喹啉 - 3 - 甲酰胺（LS 2616）是一种具有广泛作用的免疫刺激剂，对雌性BALB/c小鼠柯萨奇病毒B3诱导的心肌炎具有抗炎作用。接种后7天，这种感染在心肌中引起广泛的炎症和坏死性病变（占组织切片面积的6.8%）。当在接种前7天开始连续14天给予LS 2616时，受损面积减小（至3.7%，p<0.05），致死率降低。一种新开发的免疫组织化学染色技术阐明了心肌炎性病变中淋巴细胞亚群的原位反应模式。LS 2616使表达II类分子的细胞数量增加了3倍（p<0.0I），CTL、Ts:Th细胞比例增加了55%（p<0.05），而Lyt-1 +和TIB +细胞未受影响。LS 2616治疗7天后，脾淋巴细胞活性有增加趋势（T细胞增加21%，无统计学意义；B细胞增加60%，p<0.05）。NK细胞活性增加了51%（p<0.01）。因此，LS 2616在治疗人类炎性疾病如心肌炎方面可能具有潜力。
