Agrawal A, Pandey V C, Kumar S, Sagar P
Department of Biochemistry, Central Drug Research Institute, Lucknow, India.
J Protozool. 1989 Jan-Feb;36(1):90-2. doi: 10.1111/j.1550-7408.1989.tb02716.x.
Entamoeba histolytica (NIH-200) secreted large amounts of acid phosphatase in its external environment when grown axenically in modified TPS-II medium. Fractionation by DEAE-cellulose chromatography of the precipitate obtained from the cell-free medium at 60% ammonium sulfate saturation yielded 3 distinct peaks of enzyme activity. The enzyme in all the peaks showed resistance to tartrate but was inhibited by fluoride, cupric chloride, ethylene diamine-tetra acetic acid, ammonium molybdate and cysteine; however, enzyme associated with different peaks differed in its polyacrylamide gel electrophoretic profiles and behavior towards concanavalin A.
溶组织内阿米巴（NIH - 200）在改良的TPS - II培养基中无菌培养时，会在其外部环境中分泌大量酸性磷酸酶。对在60%硫酸铵饱和度下从无细胞培养基中获得的沉淀物进行DEAE - 纤维素柱层析分离，得到了3个不同的酶活性峰。所有峰中的酶对酒石酸盐具有抗性，但受到氟化物、氯化铜、乙二胺四乙酸、钼酸铵和半胱氨酸的抑制；然而，与不同峰相关的酶在聚丙烯酰胺凝胶电泳图谱以及对伴刀豆球蛋白A的反应方面存在差异。
