Guimaráes S, Urdaneta H, Silva E F, Tavares C A
Department of Parasitology, Federal Minas Gerais University, Belo Horizonte, Brazil.
Rev Inst Med Trop Sao Paulo. 1991 Jan-Feb;33(1):6-11. doi: 10.1590/s0036-46651991000100002.
Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypeptides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.
来自在巴西无菌分离培养的溶组织内阿米巴菌株（ICB - CSP、ICB - 462和ICB - 32）的滋养体用于制备免疫血清，并通过电泳和糖蛋白谱对其抗原进行表征，同时与在美国无菌条件下分离保存的标准菌株（HK - 9）进行比较。获得了对同源和异源抗原具有高抗体滴度的超免疫血清。所研究的四个菌株显示出相似且复杂的电泳和糖蛋白谱，显示出分子量范围从200至小于29 kDa的多肽。在致病菌株和非致病菌株之间未检测到显著差异。
