Yang Yuting, Fu Zhiwei, Su Yachun, Zhang Xu, Li Guoyin, Guo Jinlong, Que Youxiong, Xu Liping
Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University, Ministry of Agriculture, Fuzhou 350002, China.
Sci Rep. 2014 Nov 18;4:7090. doi: 10.1038/srep07090.
As one of the key enzymes in the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH) plays a role in response to abiotic stresses and pathogenesis. Here, a full-length cDNA was obtained, designed as ScG6PDH from sugarcane. The ScG6PDH gene is 1,646 bp long with a 1,524-bp long ORF encoding 507 amino acid residues. Analysis of a phylogenetic tree indicated that this gene is a member of the cytosolic G6PDH gene family, which is consistent with results from a subcellular localization experiment. Based on a real-time quantitative RT-PCR performed under salt, drought, heavy metal (CdCl2) and low temperature (4 °C) treatments, the transcription levels of the ScG6PDH gene were higher compared with transcription levels where these treatments were not imposed, suggesting a positive response of this gene to these environmental stresses. Furthermore, G6PDH activity was stimulated under 4 °C, CdCl2, NaCl and PEG treatments, but the increments varied with treatment and sampling time, implying positive response to abiotic stresses, similar to the transcript of the G6PDH gene. Ion conductivity measurements and a histochemical assay provided indirect evidence of the involvement of the ScG6PDH gene in defense reactions to the above-mentioned abiotic stresses.
作为氧化戊糖磷酸途径中的关键酶之一，葡萄糖-6-磷酸脱氢酶（G6PDH）在应对非生物胁迫和发病机制中发挥作用。在此，获得了一个全长cDNA，将其命名为甘蔗的ScG6PDH。ScG6PDH基因长1646 bp，开放阅读框长1524 bp，编码507个氨基酸残基。系统发育树分析表明，该基因是胞质G6PDH基因家族的成员，这与亚细胞定位实验的结果一致。基于在盐、干旱、重金属（CdCl2）和低温（4℃）处理下进行的实时定量RT-PCR，ScG6PDH基因的转录水平比未施加这些处理时的转录水平更高，表明该基因对这些环境胁迫有积极响应。此外，在4℃、CdCl2、NaCl和PEG处理下，G6PDH活性受到刺激，但增加量随处理和采样时间而变化，这意味着对非生物胁迫有积极响应，类似于G6PDH基因的转录本。离子电导率测量和组织化学分析为ScG6PDH基因参与对上述非生物胁迫的防御反应提供了间接证据。
