Lewis A G, Klapper P E, Cleator G M, Templeton P P, Longson M
Dept of Medical Microbiology, University of Manchester, Medical School, UK.
Res Virol. 1989 Jan-Feb;140(1):7-13. doi: 10.1016/s0923-2516(89)80078-0.
The semi-synthetic epipodophyllotoxin derivative, etoposide (VP-16-213), has been shown to inhibit nucleoside uptake in mammalian cells. The present study examined whether etoposide (or the solvent in which it is usually supplied) affected the uptake of the radioiodinated antiviral nucleoside analogue E-5-2-125Iodovinyl-2'-deoxyuridine (125IVDU) by HSV1-infected cells (human herpesvirus 1; herpesvirus simplex type 1). Etoposide was found to significantly reduce 125IVDU sequestration, although some of this effect could be attributed to the solvent. The results are discussed in relation to the use of etoposide in the development of a specific, scintigraphic brain imaging technique to enable early diagnosis of herpes encephalitis.
半合成表鬼臼毒素衍生物依托泊苷（VP - 16 - 213）已被证明可抑制哺乳动物细胞中的核苷摄取。本研究检测了依托泊苷（或其常用溶剂）是否会影响单纯疱疹病毒1型（HSV1；人疱疹病毒1型）感染细胞对放射性碘化抗病毒核苷类似物E - 5 - 2 - 125碘乙烯基 - 2'-脱氧尿苷（125IVDU）的摄取。发现依托泊苷可显著降低125IVDU的摄取，不过这种作用部分可归因于溶剂。结合依托泊苷在开发用于早期诊断疱疹性脑炎的特异性闪烁脑成像技术中的应用对结果进行了讨论。
