Dmitryjuk Małgorzata, Łopieńska-Biernat Elżbieta, Zaobidna Ewa Anna
Biochemistry Department, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowski 1A Street, 10-719 Olsztyn, Poland.
ScientificWorldJournal. 2014;2014:936560. doi: 10.1155/2014/936560. Epub 2014 Oct 27.
The in vitro effect of ivermectin lethal dose on the activity of trehalose-6-phosphate synthase (TPS) and phosphatase (TPP) and the expression of their mRNA (tps1, tps2, and tpp genes) in the muscle of adult female Ascaris suum was investigated. The presence of ivermectin in the medium caused a decrease in TPS and TPP activities during the experiment compared with the start and control groups. The exception was the group of worms grown for 8 hours in a IVM solution, in which there was a little higher TPS activity than in the control. Real-time qPCR analysis showed reduced expression of tps1 and tps2, and unchanged tpp expression after 20 hours of incubation relative to the expression at time zero. Relative to the appropriate control groups, the expression of tps2 gene was slight increased but the other two genes were reduced after 8-hours of IVM-treatment. Then the expression of all three genes was lower at the end of cultivation. The level of gene expression was positively correlated with the activity of specific enzymes. In the case of tpp gene there was only a weak correlation. Prolonged exposure to ivermectin was effective in lowering TPS and TPP activity and their mRNA expression. However, the drug did not block the pathway.
研究了伊维菌素致死剂量对成年雌性猪蛔虫肌肉中海藻糖-6-磷酸合酶（TPS）和磷酸酶（TPP）活性及其mRNA（tps1、tps2和tpp基因）表达的体外影响。与起始组和对照组相比，培养基中伊维菌素的存在导致实验期间TPS和TPP活性降低。例外的是在伊维菌素溶液中培养8小时的蠕虫组，其中TPS活性略高于对照组。实时定量PCR分析显示，相对于零时间的表达，孵育20小时后tps1和tps2的表达降低，而tpp表达不变。相对于相应的对照组，伊维菌素处理8小时后tps2基因的表达略有增加，但其他两个基因的表达降低。然后在培养结束时所有三个基因的表达都较低。基因表达水平与特定酶的活性呈正相关。就tpp基因而言，只有微弱的相关性。长时间暴露于伊维菌素可有效降低TPS和TPP活性及其mRNA表达。然而，该药物并未阻断该途径。
