Imai Y, Yamakawa M, Sato T, Suda A
Second Department of Pathology, Yamagata University School of Medicine, Japan.
Virchows Arch A Pathol Anat Histopathol. 1989;414(4):285-98. doi: 10.1007/BF00734082.
Ultrastructural, enzyme histochemical and immunohistochemical studies were performed on tissue obtained from eight cases of malignant fibrous histiocytoma (MFH) and five cases of sacral decubitus ulcer. The MFH was composed of two major tumour cell types: fibroblast-like and histiocyte-like cells. Both cell types demonstrated abundant branching, fragmented rough endoplasmic reticulum (rER), many free ribosomes, occasional small mitochondria, an oval, elliptical or irregularly shaped nucleus with one or two prominent nucleoli and often a few dense bodies. However, pseudopodial projections, multivesicular bodies and phagosomes, common histiocyte organelles, were not seen. With little difference between cases or selection sites, the MFH cells reacted to acid phosphatase (AcP) and alpha-naphtyl butyrate esterase (ANBE) by enzyme histochemistry and with ferritin (Fer), alpha 1-antitrypsin (AT), alpha 1-antichymotrypsin (ACT), fibronectin (FN), HLA-DR, HLA-DP, Leu 10 and OKT 9 in immunohistochemical studies. MFH tumour cells did not immunostain with monocyte/macrophage markers (Leu M1, Leu M3, Mo 1, Mo 2 and Macrophage) although non-neoplastic histiocytes did react to these markers. In addition, granulation tissue, such as that found in sacral decubitus ulcers, was examined and the existence of a specific cell type called the "fibrohistiocytoid (FH) cell" was documented. The FH cell was short, spindle shaped and elliptical. Ultrastructurally, it had fragmented rER distributed in a branching pattern, dispersed free ribosomes, small mitochondria and a few dense bodies, but lacked diverse fused lysosomes and distinct pseudopodial cytoplasmic extensions. The FH cells reacted with AcP, alkaline phosphatase and ANBE but not with peroxidase using enzyme histochemistry and with Fer, AT, ACT, FN, HLA-DR, HLA-DP, Leu 10 and OKT 9 but not with monocyte/macrophage markers, C3d receptor, C3bi receptor in immunohistochemical studies. The FH cells had morphological, enzyme histochemical and immunohistochemical characteristics intermediate between fibroblasts and histiocytes. Similarities between MFH cells and the FH cells seen in chronic inflammation are discussed.
对8例恶性纤维组织细胞瘤（MFH）和5例骶部褥疮溃疡组织进行了超微结构、酶组织化学和免疫组织化学研究。MFH由两种主要肿瘤细胞类型组成：成纤维细胞样细胞和组织细胞样细胞。两种细胞类型均显示丰富的分支状、破碎的粗面内质网（rER）、许多游离核糖体、偶尔可见的小线粒体、一个椭圆形、椭圆形或不规则形的细胞核，有一个或两个明显的核仁，且常有一些致密小体。然而，未见假足样突起、多囊泡体和吞噬体等常见的组织细胞细胞器。不同病例或取材部位之间差异不大，MFH细胞在酶组织化学中对酸性磷酸酶（AcP）和α-萘丁酸酯酶（ANBE）有反应，在免疫组织化学研究中对铁蛋白（Fer）、α1-抗胰蛋白酶（AT）、α1-抗糜蛋白酶（ACT）、纤连蛋白（FN）、HLA-DR、HLA-DP、Leu 10和OKT 9有反应。MFH肿瘤细胞用单核细胞/巨噬细胞标志物（Leu M1、Leu M3、Mo 1、Mo 2和巨噬细胞）免疫染色呈阴性，而非肿瘤性组织细胞对这些标志物有反应。此外，对骶部褥疮溃疡中发现的肉芽组织进行了检查，并记录了一种称为“纤维组织细胞样（FH）细胞”的特定细胞类型的存在。FH细胞短，呈纺锤形和椭圆形。超微结构上，它有呈分支状分布的破碎rER、分散的游离核糖体、小线粒体和一些致密小体，但缺乏各种融合的溶酶体和明显的假足样细胞质延伸。在酶组织化学中，FH细胞对AcP、碱性磷酸酶和ANBE有反应，但对过氧化物酶无反应；在免疫组织化学研究中，对Fer、AT、ACT、FN、HLA-DR、HLA-DP、Leu 10和OKT 9有反应，但对单核细胞/巨噬细胞标志物、C3d受体、C3bi受体无反应。FH细胞具有介于成纤维细胞和组织细胞之间的形态学、酶组织化学和免疫组织化学特征。讨论了MFH细胞与慢性炎症中所见FH细胞之间的相似性。
