Juang Chi-Long, Yang Fei Shish, Hsieh Ming Shuang, Tseng Hu-Yi, Chen Su-Chiu, Wen Hsiao-Chuan
Department of Optometry, Yuanpei University, Hsinchu 30015, Taiwan.
BMC Complement Altern Med. 2014 Nov 18;14:447. doi: 10.1186/1472-6882-14-447.
Spinal cord injury (SCI)-induced secondary oxidative stress associates with a clinical complication and high mortality. Treatments to improve the neurological outcome of secondary injury are considered as important issues. The objective of the current study is to evaluate the anti-oxidative effect of Tithonia diversifolia ethanolic extracts (TDE) on cells and apply the pharmacological effect to SCI model using a MRI imaging algorism.
The anti-oxidation properties were tested in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Rat liver cells (clone-9) were treated with various doses of TDE (0 ~ 50 μg/ml) before exposed to 250 μM H2O2 and cell survival was determined by MTT and LDH assays. We performed water apparent diffusion coefficient (ADC) map in MR techniques to investigate the efficacy of TDE treatment on SCI animal model. We performed T5 laminectomy and compression (50 g, 1 min) to induce SCI. PHILIP 3.0 T MRI was used to image 24 male Sprague-Dawley rats weighing 280-320 g. Rats were randomly divided into three groups: sham group, SCI group, SCI treated with TDE group. The MRI images were taken and ADC were acquired before and after of treatment of TDE (50 mg/kg B. W. orally, 5 days) in SCI model.
TDE protected clone-9 cells against H2O2-induced toxicity through DPPH scavenging mechanism. In addition, SCI induced the increase in ADC after 6 hours. TDE treatment slightly decreased the ADC level after 1-week SCI compared with control animals.
Our studies have proved that the cytoprotection effect of TDE, at least in part, is through scavenging ROS to eliminate intracellular oxidative stress and highlight a potential therapeutic consideration of TDE in alternative and complementary medicine.
脊髓损伤（SCI）诱导的继发性氧化应激与临床并发症及高死亡率相关。改善继发性损伤神经学结果的治疗方法被视为重要问题。本研究的目的是评估肿柄菊乙醇提取物（TDE）对细胞的抗氧化作用，并使用磁共振成像算法将其药理作用应用于SCI模型。
在2,2-二苯基-1-苦基肼（DPPH）自由基清除试验中测试抗氧化性能。大鼠肝细胞（clone-9）在暴露于250μM过氧化氢之前用不同剂量的TDE（0至50μg/ml）处理，并通过MTT和LDH试验测定细胞存活率。我们在磁共振技术中进行水表观扩散系数（ADC）图，以研究TDE治疗对SCI动物模型的疗效。我们进行T5椎板切除术并压迫（50g，1分钟）以诱导SCI。使用飞利浦3.0T磁共振成像对24只体重280-320g的雄性Sprague-Dawley大鼠进行成像。大鼠随机分为三组：假手术组、SCI组、TDE治疗的SCI组。在SCI模型中，在TDE治疗（50mg/kg体重，口服，5天）前后采集磁共振图像并获取ADC值。
TDE通过DPPH清除机制保护clone-9细胞免受过氧化氢诱导的毒性。此外，SCI在6小时后诱导ADC增加。与对照动物相比，TDE治疗在SCI 1周后略微降低了ADC水平。
我们的研究证明，TDE的细胞保护作用至少部分是通过清除活性氧来消除细胞内氧化应激，并突出了TDE在替代医学和补充医学中的潜在治疗价值。
