Worden N, Esteve V Esteva, Domozych D S, Drakakaki G
Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA.
Methods Mol Biol. 2015;1242:23-39. doi: 10.1007/978-1-4939-1902-4_2.
The cell wall is directly involved in cell growth, and its ability to loosen and rearrange allows for cell expansion through the existing turgor pressure. Thus, information on cell wall deposition and rearrangement can provide insights into the overall plant growth. This chapter describes two methods that can be used to evaluate cell expansion (1) in the model plant Arabidopsis thaliana and (2) the model alga Penium margaritaceum. These methods are further used to screen for small molecules that induce cell growth phenotypic changes affecting cell wall. Identification of such small molecules is beneficial due to their posttranslational mechanism of action that can be controlled in a temporal and spatial manner. Chemical genomics has the ability to overcome issues of genetic redundancy and lethality, which can hinder traditional genetic methods. The identification of small molecules in these screens will provide useful information on plant cell wall biology and overall plant growth.
细胞壁直接参与细胞生长，其松弛和重排的能力使得细胞能够通过现有的膨压进行扩张。因此，有关细胞壁沉积和重排的信息可以为了解植物整体生长提供线索。本章介绍两种可用于评估细胞扩张的方法：（1）在模式植物拟南芥中，以及（2）在模式藻类玛格丽塔扁藻中。这些方法进一步用于筛选能够诱导影响细胞壁的细胞生长表型变化的小分子。鉴定此类小分子是有益的，因为它们的翻译后作用机制可以在时间和空间上得到控制。化学基因组学有能力克服可能阻碍传统遗传方法的遗传冗余和致死性问题。在这些筛选中鉴定出的小分子将为植物细胞壁生物学和植物整体生长提供有用信息。
