Zheng Bingbing, Xue Xiangyang, Zhao Yiping, Chen Jing, Xu Chao-Yi, Duan Ping
Department of Obstetrics, First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.
Department of Microbiology, Wenzhou Medical University, Zhejiang, China.
Iran J Reprod Med. 2014 Aug;12(8):555-60.
Recent studies showed that inappropriate expression of microRNAs (miRNAs) is strongly associated with tumor-related processes in humans (2-9,11-17).
To understand the changes of miRNAs in endometriosis.
With real-time RT-PCR, we investigated the miR-143 and miR-145 expression in eutopic (EU, n=2) and ectopic endometrium (EC, n=11) (from women with endometriosis) (as well as EU+EC, n=11), along with the normal endometrium (EN, n=22) (from women without endometriosis, but with leiomyoma).
We did not find that the expression of miR-143 and/or miR-145 in EN or EC changed with menstrual cycle. But our results showed the miR-143 was up-regulated in EC (p=0.000) compared to EN. The miR-143 was also up-regulated in EU, but the difference did not reach statistically significance (p=0.053). Compared to EU, the expression of miR-143 in EC was up-regulated (p=0.016). MiR-145 had the similar characteristic to miR-143. The miR-145 was up-regulated in both EU (p=0.004) and EC (p=0.000) in compared to EN group. When compared with EU, the miR-145 in EC was up-regulated (p=0.008).
In conclusion, the miR-143 and miR-145 may play a certain role in the development and progression of endometriosis.
近期研究表明，微小RNA（miRNA）的异常表达与人类肿瘤相关进程密切相关（2 - 9,11 - 17）。
了解子宫内膜异位症中miRNA的变化。
采用实时逆转录聚合酶链反应（RT-PCR），我们检测了在位内膜（EU，n = 2）、异位内膜（EC，n = 11）（来自子宫内膜异位症患者）以及EU + EC（n = 11）中miR - 143和miR - 145的表达，同时检测了正常子宫内膜（EN，n = 22）（来自无子宫内膜异位症但有平滑肌瘤的女性）。
我们未发现EN或EC中miR - 143和/或miR - 145的表达随月经周期变化。但我们的结果显示，与EN相比，EC中miR - 143上调（p = 0.000）。EU中miR - 143也上调，但差异未达到统计学意义（p = 0.053）。与EU相比，EC中miR - 143的表达上调（p = 0.016）。miR - 145具有与miR - 143相似的特征。与EN组相比，EU（p = 0.004）和EC（p = 0.000）中miR - 145均上调。与EU相比，EC中miR - 145上调（p = 0.008）。
总之，miR - 143和miR - 145可能在子宫内膜异位症的发生发展中起一定作用。
