Hajimaqsoudi Elnaz, Darbeheshti Farzaneh, Kalantar Seyed Mehdi, Javaheri Atiyeh, Mirabutalebi Seyed Hamidreza, Sheikhha Mohammad Hasan
Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Int J Reprod Biomed. 2020 Oct 13;18(10):825-836. doi: 10.18502/ijrm.v13i10.7767. eCollection 2020 Oct.
Endometriosis is generally considered as a benign condition; however, there is a possibility for it to become cancerous. miR-125b is upregulated in both endometriotic tissues and serum samples of women with endometriosis but its potential targets in endometriosis are still not fully understood.
The role of miR-125b in the regulation of expression in endometriosis was tested with a bioinformatics approach. In addition, the expression of miR-125b and in both eutopic (Eu-p) and ectopic endometrium (Ec-p) in the endometrium tissues of women with endometriosis was compared to those in the normal endometrium tissues of controls (Normal).
In this case-control study, the Eu-p and Ec-p samples were collected from 20 women who underwent laparoscopic surgery, and the normal endometrium tissues were collected from 20 controls with no evidence of endometriosis. For bioinformatics approach, a protein-protein interaction network was constructed based on co-expressed potential targets of miR-125b. Quantitative polymerase chain reaction technique was used for the measurement of miR125b and expression.
Our results showed that miR-125b was significantly overexpressed in Ec-p (p-value: 0.021). In addition, there was a significant under expression in both the Ec-p and Eu-p samples compared with the Normal tissues (p-value: 0.003).
The negative correlation between miR-125b and as well as a noticeable decreased expression of in both Ec-p and Eu-p samples may be interpreted as the roles of miR-125b/ axis in the pathogenesis of endometriosis. In addition, these findings and bioinformatic analyses imply a possible role of miR-125b in cancer-like features of endometriosis.
子宫内膜异位症通常被认为是一种良性疾病；然而，它有癌变的可能性。在子宫内膜异位症患者的异位内膜组织和血清样本中，miR-125b均呈上调状态，但其在子宫内膜异位症中的潜在靶点仍未完全明确。
采用生物信息学方法检测miR-125b在子宫内膜异位症中对基因表达的调控作用。此外，比较子宫内膜异位症患者子宫内膜组织中在位内膜（Eu-p）和异位内膜（Ec-p）中miR-125b和基因的表达与正常对照组（Normal）内膜组织中的表达情况。
在这项病例对照研究中，从20例行腹腔镜手术的女性中收集Eu-p和Ec-p样本，从20例无子宫内膜异位症证据的对照者中收集正常子宫内膜组织。对于生物信息学方法，基于miR-125b的共表达潜在靶点构建蛋白质-蛋白质相互作用网络。采用定量聚合酶链反应技术检测miR-125b和基因的表达。
我们的结果显示，miR-125b在Ec-p中显著过表达（p值：0.021）。此外，与正常组织相比，Ec-p和Eu-p样本中的基因表达均显著下调（p值：0.003）。
miR-125b与基因之间的负相关以及Ec-p和Eu-p样本中基因表达的显著降低可能表明miR-125b/基因轴在子宫内膜异位症发病机制中的作用。此外，这些发现和生物信息学分析暗示了miR-125b在子宫内膜异位症类癌特征中的可能作用。
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