Gonzaga L L, Costa L E O, Santos T T, Araújo E F, Queiroz M V
Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.
J Appl Microbiol. 2015 Feb;118(2):485-96. doi: 10.1111/jam.12696. Epub 2014 Dec 29.
To evaluate the diversity of endophytic fungi from the leaves of the common bean and the genetic diversity of endophytic fungi from the genus Colletotrichum using IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism) analyses.
The fungi were isolated by tissue fragmentation and identified by analysing the morphological features and sequencing the internal transcribed spacer (ITS) regions and the rDNA large subunit (LSU). Twenty-seven different taxa were identified. Colletotrichum was the most commonly isolated genera from the common bean (32.69% and 24.29% of the total isolates from the Ouro Negro and Talismã varieties, respectively). The IRAP and REMAP analyses revealed a high genetic diversity in the Colletotrichum endophytic isolates and were able to discriminate these isolates from the phytopathogen Colletotrichum lindemuthianum.
Fungi from the genus Colletotrichum are abundant in the Phaseolus vulgaris endophytic community, and the IRAP and REMAP markers can be used to rapidly distinguish between C. lindemuthianum and other Colletotrichum members that are frequently found as endophytes.
This is the first report of the diversity of endophytic fungi present in the common bean and the use of IRAP and REMAP markers to assess the genetic diversity of endophytic fungi from the genus Colletotrichum.
利用IRAP（反转录转座子间扩增多态性）和REMAP（反转录转座子-微卫星扩增多态性）分析，评估普通豆叶片内生真菌的多样性以及炭疽菌属内生真菌的遗传多样性。
通过组织破碎分离真菌，并通过分析形态特征以及对内部转录间隔区（ITS）和核糖体DNA大亚基（LSU）进行测序来鉴定。共鉴定出27个不同的分类单元。炭疽菌是从普通豆中最常分离出的属（分别占来自奥罗内格罗和塔利斯马品种的总分离株的32.69%和24.29%）。IRAP和REMAP分析揭示了炭疽菌内生分离株具有高度的遗传多样性，并且能够将这些分离株与植物病原菌林氏炭疽菌区分开来。
炭疽菌属真菌在菜豆内生菌群落中丰富存在，并且IRAP和REMAP标记可用于快速区分林氏炭疽菌和其他常见的作为内生菌的炭疽菌属成员。
这是关于普通豆中存在的内生真菌多样性以及使用IRAP和REMAP标记评估炭疽菌属内生真菌遗传多样性的首次报道。
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