Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, MG, CEP 36571-000, Brazil.
World J Microbiol Biotechnol. 2012 Mar;28(3):1087-95. doi: 10.1007/s11274-011-0909-x. Epub 2011 Oct 1.
A nonautonomous element of 624 bp, called RetroCl1 (Retroelement Colletotrichum lindemuthianum 1), was identified in the plant pathogenic fungus Colletotrichum lindemuthianum. RetroCl1 contains terminal direct repeats (223 bp) that are surrounded by CTAGT sequences. It has a short internal domain of 178 bp and shows characteristics of terminal-repeat retrotransposon in miniature (TRIM) family. We used RetroCl1 sequence to develop molecular markers for the Colletotrichum genus. IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism) markers were used to analyze the genetic diversity of C. lindemuthianum. Fifty-four isolates belonging to different races were used. A total of 45 loci were amplified. The Nei index showed significant differences among the populations divided according to race, indicating that they are structured according to pathotype. No clear correlation between IRAP and REMAP markers with pathogenic characterization was found. C. lindemuthianum has high genetic diversity, and the analysis of molecular variance showed that 51% of variability is found among the populations of different races. The markers were also tested in different Colletotrichum species. In every case, multiple bands were amplified, indicating that these markers can be successfully used in different species belonging to the Colletotrichum genus.
一个非自主元件的 624bp，称为 RetroCl1（ Retroelement Colletotrichum lindemuthianum 1），在植物病原真菌胶孢炭疽菌中被鉴定出来。RetroCl1 包含末端直接重复序列（223bp），周围是 CTAGT 序列。它有一个短的内部结构域 178bp，并显示出微型末端重复转座子（TRIM）家族的特征。我们使用 RetroCl1 序列为胶孢炭疽菌属开发了分子标记。IRAP（Inter-Retrotransposon Amplified Polymorphism）和 REMAP（Retrotransposon-Microsatellite Amplified Polymorphism）标记被用于分析胶孢炭疽菌的遗传多样性。使用了属于不同小种的 54 个分离物。共扩增了 45 个位点。根据小种划分的种群的 Nei 指数显示出显著差异，表明它们是根据致病型结构的。IRAP 和 REMAP 标记与致病特征之间没有发现明显的相关性。胶孢炭疽菌具有很高的遗传多样性，分子方差分析表明，不同小种种群之间的变异性为 51%。这些标记也在不同的胶孢炭疽菌种中进行了测试。在每种情况下，都扩增了多个条带，表明这些标记可以成功地用于属于胶孢炭疽菌属的不同物种。
