Gupta V, Gulati P, Bhagat N, Dhar M S, Virdi J S
Medical Microbiology and Bioprocess Technology Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, India.
Eur J Clin Microbiol Infect Dis. 2015 Apr;34(4):641-50. doi: 10.1007/s10096-014-2276-7. Epub 2014 Nov 20.
Yersinia enterocolitica is a gastrointestinal pathogen which causes yersiniosis, an illness characterized by diarrhea, ileitis, and mesenteric lymphadenitis. Y. enterocolitica is transmitted via the feco-oral route by the consumption of contaminated food or water. Several phenotypic and genotypic methods have been developed to reliably detect Y. enterocolitica in food. However, the source of infection of many recently reported foodborne outbreaks remains obscure. The detection of this pathogen in food is a challenging task, since it shares similarities with other enteric bacteria. The presence of other microorganisms in the food samples makes it even more difficult to identify this slow-growing pathogen. Therefore, the present-day emphasis is on the development of sensitive, easily automated methods suitable for in-situ detection, allowing quick and cost-effective characterization of food samples. This review summarizes and compares the currently available cultural, immunological, and molecular methods, particularly in relation to their specific merits or demerits when implemented for the detection of Y. enterocolitica in food.
小肠结肠炎耶尔森菌是一种胃肠道病原体，可引起耶尔森菌病，该病的特征为腹泻、回肠炎和肠系膜淋巴结炎。小肠结肠炎耶尔森菌通过食用受污染的食物或水经粪口途径传播。已经开发了几种表型和基因型方法来可靠地检测食品中的小肠结肠炎耶尔森菌。然而，许多最近报告的食源性疾病暴发的感染源仍不明确。在食品中检测这种病原体是一项具有挑战性的任务，因为它与其他肠道细菌有相似之处。食品样本中其他微生物的存在使得识别这种生长缓慢的病原体更加困难。因此，目前的重点是开发适用于原位检测的灵敏、易于自动化的方法，以便对食品样本进行快速且经济高效的表征。本综述总结并比较了目前可用的培养、免疫和分子方法，特别是在用于检测食品中小肠结肠炎耶尔森菌时它们的具体优缺点。
