Bucher Michael, Meyer Cornelia, Grötzbach Britta, Wacheck Silke, Stolle Andreas, Fredriksson-Ahomaa Maria
Bayerisches Landesamt fuer Gesundheit und Lebensmittelsicherheit, Oberschleissheim, Germany.
Foodborne Pathog Dis. 2008 Jun;5(3):273-80. doi: 10.1089/fpd.2007.0076.
Yersinia enterocolitica is the most common species causing enteric yersiniosis, which is still the third most frequently reported foodborne gastroenteritis in Europe. Y. enterocolitica generally causes sporadic human infections, and outbreaks are rare. The most important infection source of yersiniosis is believed to be contaminated pork and pork products. Data on the prevalence of pathogenic Y. enterocolitica in animals and foodstuffs are very limited and old; thus, more information on the extent and range of the prevalence of this enteropathogen in nonhuman sources is needed. In this work, prevalence of pathogenic Y. enterocolitica in different sources in Bavaria is presented. Further, the antimicrobial resistance of human and nonhuman strains is reported. The highest isolation rate of pathogenic Y. enterocolitica (67%) was found in tonsils of slaughter pigs. No pathogenic strains were isolated from cattle, sheep, turkey, and horses. ail-Positive Y. enterocolitica was detected in dogs (5%), cats (3%), and rodents (3%) by real-time PCR. Pathogenic Y. enterocolitica was isolated only from raw pork, especially from edible offal (51%). Surprisingly, 38% of game was contaminated with this pathogen when the samples were studied with PCR. Additionally, some raw pork sausages and one poultry sample were PCR positive. All pathogenic Y. enterocolitica isolates from nonhuman sources were belonging to bioserotype 4/O:3. Antimicrobial resistance of 60 human and 140 porcine strains of bioserotype 4/O:3 was tested by the agar disc diffusion method to 15 different antimicrobial agents. All Y. enterocolitica 4/O:3 strains were susceptible to most of the tested antibacterial agents.
小肠结肠炎耶尔森菌是引起肠道耶尔森菌病最常见的菌种，在欧洲，肠道耶尔森菌病仍是报告频率第三高的食源性肠胃炎。小肠结肠炎耶尔森菌通常引起散发性人类感染，暴发情况罕见。耶尔森菌病最重要的感染源据信是受污染的猪肉和猪肉制品。关于致病性小肠结肠炎耶尔森菌在动物和食品中的流行数据非常有限且陈旧；因此，需要更多关于这种肠道病原体在非人类来源中的流行程度和范围的信息。在这项研究中，展示了巴伐利亚不同来源中致病性小肠结肠炎耶尔森菌的流行情况。此外，还报告了人类和非人类菌株的抗菌药物耐药性。致病性小肠结肠炎耶尔森菌的最高分离率（67%）在屠宰猪的扁桃体中发现。未从牛、羊、火鸡和马中分离出致病菌株。通过实时PCR在狗（5%）、猫（3%）和啮齿动物（3%）中检测到了呈阳性的小肠结肠炎耶尔森菌。致病性小肠结肠炎耶尔森菌仅从生猪肉中分离出来，尤其是从可食用内脏（51%）中分离出来。令人惊讶的是，当用PCR研究样本时，38%的野味被这种病原体污染。此外，一些生猪肉香肠和一个家禽样本PCR呈阳性。所有来自非人类来源的致病性小肠结肠炎耶尔森菌分离株均属于生物血清型4/O:3。采用琼脂纸片扩散法对60株人类和140株生物血清型4/O:3的猪源菌株进行了15种不同抗菌药物的抗菌药物耐药性测试。所有小肠结肠炎耶尔森菌4/O:3菌株对大多数测试抗菌药物敏感。
