通过结合去除丰富蛋白质和多凝集素亲和色谱法开发改进的人血浆蛋白质组分级分离方法。
Development of an improved fractionation of the human plasma proteome by a combination of abundant proteins depletion and multi-lectin affinity chromatography.
作者信息
Gbormittah Francisca O, Hincapie Marina, Hancock William S
机构信息
Barnett Institute & Department of Chemistry & Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
出版信息
Bioanalysis. 2014;6(19):2537-48. doi: 10.4155/bio.14.217.
AIM
Current analytical tools lack the required capacity to reduce the complexity of the plasma proteome and identify low-level proteins of clinical interest. Hence, the need to develop a fractionation approach to provide adequate throughput for a clinical study and minimize the loss and improve the detection of low abundance proteins.
MATERIALS & METHODS: We present the development of an analytical platform that combines the depletion of 12 high abundance proteins and multi-lectin affinity chromatography (12P-M-LAC) fractionation.
RESULTS & CONCLUSION: We validated the highly specific, stable and robust 12P-M-LAC platform using human plasma. An improved enrichment of low abundance proteins and glycoproteins with minimum sample loss was achieved demonstrating the suitability of this platform in future biomarker discovery studies.
目的
当前的分析工具缺乏降低血浆蛋白质组复杂性以及鉴定具有临床意义的低丰度蛋白质所需的能力。因此，需要开发一种分级分离方法，以提供足够的通量用于临床研究，并最大限度地减少损失，同时改善低丰度蛋白质的检测。
材料与方法
我们展示了一种分析平台的开发，该平台结合了12种高丰度蛋白质的去除和多凝集素亲和色谱（12P-M-LAC）分级分离。
结果与结论
我们使用人血浆验证了高度特异、稳定且强大的12P-M-LAC平台。实现了低丰度蛋白质和糖蛋白的富集得到改善，且样品损失最小，证明了该平台适用于未来的生物标志物发现研究。
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