Wu Chaochao, Duan Jicheng, Liu Tao, Smith Richard D, Qian Wei-Jun
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States.
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States.
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 15;1021:57-68. doi: 10.1016/j.jchromb.2016.01.015. Epub 2016 Jan 12.
Human biofluids, especially blood plasma or serum, hold great potential as the sources of candidate biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge for detecting promising low-abundance proteins. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely applied for separating low-abundance proteins from the high- and moderate-abundance proteins, thus enabling much more effective detection of low-abundance proteins. Herein, we review the advances of immunoaffinity separation methods and their contributions to the proteomic applications in human biofluids. The limitations and future perspectives of immunoaffinity separation methods are also discussed.
人体生物流体，尤其是血浆或血清，作为各种疾病候选生物标志物的来源具有巨大潜力；然而，生物流体中蛋白质浓度的巨大动态范围对检测有前景的低丰度蛋白质构成了重大分析挑战。在过去十年中，已开发出各种免疫亲和色谱方法并常规用于从高丰度和中等丰度蛋白质中分离低丰度蛋白质，从而能够更有效地检测低丰度蛋白质。在此，我们综述免疫亲和分离方法的进展及其对人体生物流体蛋白质组学应用的贡献。还讨论了免疫亲和分离方法的局限性和未来前景。
