Shivaswamy VinodKumar Chickmangalure, Kalasuramath Suneeta Basavaraj, Sadanand Chethan Kumar, Basavaraju Abhishek Kilagere, Ginnavaram Varsha, Bille Sumanth, Ukken Sanjay Saju, Pushparaj Usha Nandini
1 Department of Microbiology, S.S. Institute of Medical Sciences and Research Centre , Davangere, Karnataka, India .
Microb Drug Resist. 2015 Apr;21(2):171-7. doi: 10.1089/mdr.2014.0120. Epub 2014 Nov 20.
Acinetobacter baumannii, a substantial nosocomial pathogen, has developed resistance to almost all available antimicrobial drugs. Bacteriophage therapy is a possible alternative treatment for multidrug-resistant (MDR) bacterial infections. In this study, we have successfully isolated bacteriophage active against clinical strains of A. baumannii by enrichment from hospital sewage sludge using representatives of those strains. The bacteriophage isolated against A. baumannii formed plaques against beta-lactamases producing strains of A. baumannii. The utility of bacteriophage specific for A. baumannii to resolve wound infection in uncontrolled diabetic rats was evaluated. Five groups of uncontrolled diabetic rats were used. Group I was noninfected (Control), Group II was infected with MDR A. baumannii and challenged with bacteriophage, Group III was infected with MDR A. baumannii, Group IV was infected with MDR A. baumannii and challenged with antibiotic colistin, and Group V consisted of noninfected rats and sprayed with phage (Phage control). A significant decrease in infection, period of epithelization, and wound contraction was observed in the phage-challenged group when compared with antibiotic-treated uncontrolled diabetic rats and the control group. To conclude the study, new insights are provided into the biology of the broad host range of A. baumannii phage, demonstrating that A. baumannii phage has prospects for the treatment of infections caused by the MDR A. baumannii.
鲍曼不动杆菌是一种重要的医院病原体，已对几乎所有可用的抗菌药物产生耐药性。噬菌体疗法是治疗多重耐药（MDR）细菌感染的一种可能替代方法。在本研究中，我们通过使用鲍曼不动杆菌临床菌株的代表物从医院污水污泥中富集，成功分离出了对鲍曼不动杆菌临床菌株具有活性的噬菌体。分离得到的针对鲍曼不动杆菌的噬菌体对产生β-内酰胺酶的鲍曼不动杆菌菌株形成噬菌斑。评估了特异性针对鲍曼不动杆菌的噬菌体在解决未控制的糖尿病大鼠伤口感染中的效用。使用了五组未控制的糖尿病大鼠。第一组未感染（对照组），第二组感染多重耐药鲍曼不动杆菌并用噬菌体攻击，第三组感染多重耐药鲍曼不动杆菌，第四组感染多重耐药鲍曼不动杆菌并用抗生素黏菌素攻击，第五组由未感染大鼠并用噬菌体喷雾（噬菌体对照组）。与用抗生素治疗的未控制糖尿病大鼠和对照组相比，噬菌体攻击组的感染、上皮化期和伤口收缩均显著降低。为总结本研究，我们对鲍曼不动杆菌噬菌体广泛宿主范围的生物学特性有了新的认识，表明鲍曼不动杆菌噬菌体在治疗多重耐药鲍曼不动杆菌引起的感染方面具有前景。
