School of Science, Walailak University, Nakhon Si Thammarat 80161, Thailand.
Division of Computational Science, Faculty of Science, Prince of Songkla University, Hat Yai 90110, Thailand.
Viruses. 2022 Jan 20;14(2):194. doi: 10.3390/v14020194.
Multidrug-resistant (MDR ) is one of the ESKAPE pathogens that restricts available treatment options. MDR is responsible for a dramatic increase in case numbers of a wide variety of infections, including skin and soft tissue infections (SSTIs), resulting in pyoderma, surgical debridement, and necrotizing fasciitis. To investigate an alternative medical treatment for SSTIs, a broad range lytic phage, vB _AbP_ABWU2101 (phage vABWU2101), for lysing MDR in associated SSTIs was isolated and the biological aspects of this phage were investigated. Morphological characterization and genomic analysis revealed that phage vABWU2101 was a new species in the , , family , and order . Antibiofilm activity of phage vABWU2101 demonstrated good activity against both preformed biofilms and biofilm formation. The combination of phage vABWU2101 and tigecycline showed synergistic antimicrobial activities against planktonic and biofilm cells. Scanning electron microscopy confirmed that the antibacterial efficacy of the combination of phage vABWU2101 and tigecycline was more effective than the phage or antibiotic alone. Hence, our findings could potentially be used to develop a therapeutic option for the treatment of SSTIs caused by MDR
耐多药（MDR）是 ESKAPE 病原体之一，限制了可用的治疗选择。MDR 导致多种感染（包括皮肤和软组织感染（SSTIs））的病例数量急剧增加，导致脓皮病、手术清创和坏死性筋膜炎。为了研究 SSTIs 的替代医学治疗方法，从相关 SSTIs 中分离出一种广泛的裂解噬菌体 vB _AbP_ABWU2101（噬菌体 vABWU2101）来裂解 MDR，研究了这种噬菌体的生物学特性。形态学特征和基因组分析表明，噬菌体 vABWU2101是 科 目 属的一个新种 。噬菌体 vABWU2101 的抗生物膜活性对已形成的生物膜和生物膜形成均表现出良好的活性。噬菌体 vABWU2101 与替加环素的联合使用对浮游细胞和生物膜细胞均表现出协同抗菌活性。扫描电子显微镜证实，噬菌体 vABWU2101 与替加环素联合使用的杀菌效果比单独使用噬菌体或抗生素更有效。因此，我们的研究结果可能有助于开发治疗耐多药引起的 SSTIs 的治疗方法。
