[维生素D受体Fok I和Bsm I基因多态性与老年男性2型糖尿病患者血脂异常的相关性]

[Association of vitamin D receptor Fok I and Bsm I polymorphisms with dyslipidemias in elderly male patients with type 2 diabetes].

作者信息

Xia Zheng, Hu Yazhuo, Zhang Honghong, Han Zhitao, Bai Jie, Fu Shuhong, Deng Xinli, He Yao

机构信息

Institute of Geriatrics/Key Laboratory of Normal Aging and Geriatrics, General Hospital of PLA/Chinese PLA Medical School, Beijing 100853, China. E-mail:

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2014 Nov;34(11):1562-8.

PMID:25413050

Abstract

OBJECTIVE

To assess the association of vitamin D receptor (VDR) gene Fok I and Bsm I polymorphisms with dyslipidemia in elderly male patients with type 2 diabetes of Han nationality.

METHODS

A total of 328 elderly male residents of Han nationality in Beijing, including 237 type 2 diabetic patients and 91 healthy control subjects, were enrolled in this study. The diabetic patients were divided into non-dyslipidemia group (DO group, n=134) and dyslipidemia group (DH group, n=103). All the participants were genotyped for Fok I and Bsm I polymorphisms in VDR gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing technology, and the results were compared with their clinical characteristics.

RESULTS

For Fok I, the frequency of F allele was significantly higher in the diabetic patients than in the control group (Χ(2)=3.873, P=0.049, OR=1.439, 95% CI: 1.001-2.071). In the dominant model, the frequency of FF genotype was significantly higher in the diabetic group (Χ(2)=5.057, P=0.025, OR=1.756, 95% CI: 1.072-2.875) as well as in DH group (Χ(2)=6.168, P=0.013, OR=2.06, 95% CI: 1.161-3.663) than in the control group. There was no significant differences in the genotype frequency or allele distribution in other paired groups (P>0.05). Compared with Ff + ff genotype, FF genotype was associated with a significantly decreased average diastolic blood pressure (P=0.039) but significantly increased postprandial blood glucose (P=0.035), triglycerides (P=0.049) and uric acid (P=0.031). No significant difference was detected in genotype frequency or allele distribution of Bsm I polymorphisms between the groups (P>0.05); serum creatinine levels were significantly higher in bb genotype than in BB + Bb genotype group (P=0.011).

CONCLUSION

VDR gene Fok I polymorphisms may be a risk factor for dyslipidemia in elderly male patients with type 2 diabetes among Chinese Han population, where Bsm I polymorphisms are not associated with diabetic dyslipdiemia.

摘要

目的

评估维生素D受体（VDR）基因Fok I和Bsm I多态性与汉族老年男性2型糖尿病患者血脂异常的相关性。

方法

本研究纳入了328名北京汉族老年男性居民，其中包括237例2型糖尿病患者和91名健康对照者。将糖尿病患者分为非血脂异常组（DO组，n = 134）和血脂异常组（DH组，n = 103）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和DNA测序技术对所有参与者的VDR基因Fok I和Bsm I多态性进行基因分型，并将结果与其临床特征进行比较。

结果

对于Fok I，糖尿病患者中F等位基因频率显著高于对照组（Χ(2)=3.873，P = 0.049，OR = 1.439，95% CI：1.001 - 2.071）。在显性模型中，糖尿病组（Χ(2)=5.057，P = 0.025，OR = 1.756，95% CI：1.072 - 2.875）以及DH组（Χ(2)=6.168，P = 0.013，OR = 2.06，95% CI：1.161 - 3.663）中FF基因型频率显著高于对照组。其他配对组的基因型频率或等位基因分布无显著差异（P>0.05）。与Ff + ff基因型相比，FF基因型与平均舒张压显著降低（P = 0.039）但餐后血糖（P = 0.035）、甘油三酯（P = 0.049）和尿酸（P = 0.031）显著升高相关。各组间Bsm I多态性的基因型频率或等位基因分布无显著差异（P>0.05）；bb基因型组的血清肌酐水平显著高于BB + Bb基因型组（P = 0.011）。