Beysel Selvihan, Eyerci Nilnur, Ulubay Mustafa, Caliskan Mustafa, Kizilgul Muhammed, Hafızoğlu Merve, Cakal Erman
Department of Endocrinology and Metabolism, Ankara Diskapi Yildirim Beyazit Teaching and Training Research Hospital, Ankara, Turkey.
2Department of Medical Biology, Baskent University, Ankara, Turkey.
Diabetol Metab Syndr. 2019 May 14;11:37. doi: 10.1186/s13098-019-0434-x. eCollection 2019.
Pre-pregnancy obesity, gestational diabetes mellitus (GDM), and gestational weight gain (GWG) are associated with each other. This is the first study to investigate whether genetic variants were associated with having GDM, and whether genetic variants-related GDM were associated with adiposity including pre-pregnancy obesity and excessive GWG in Turkish women.
Women with GDM (n = 160) and without GDM (n = 145) were included in case-controlled study. Genotyping of the gene (p.I27L rs1169288, p.98V rs1800574, p.S487N rs2464196), the gene (p.BsmI rs1544410, p.ApaI rs7975232, p.TaqI rs731236, p.FokI rs2228570), and gene (rs9939609) SNPs were performed by using RT-PCR.
The AA genotype was associated with an increased risk of having GDM (AA vs. AT + TT, 24.4% vs. 12.4%, OR = 2.27, 95% CI [1.23-4.19], p = 0.007). The p.I27L GT/TT genotype was associated with increased GDM risk (GT + TT vs. GG-wild, 79.4% vs. 65.5%, OR = 2.02, 95% CI 1.21-3.38], p = 0.007). However, all gene SNPs and the p.A98V, p.S487N were not associated with having GDM (p > 0.05). The AA genotype was associated with an increased risk for pre-pregnancy overweight/obesity (OR = 1.43, 95% CI [1.25-3.4], p = 0.035), but not associated with excessive GWG after adjusting for pre-pregnancy weight (p > 0.05). Pre-pregnancy weight, weight at delivery, and GWG did not differ in both and gene carriers (p > 0.05). HOMA-IR and HbA1c were increased in both p.I27L TT and AA genotype carriers (p < 0.05).
The adiposity-related gene is associated with GDM by the effect of on pre-pregnancy obesity. The diabetes-related p.I27L gene is associated with GDM by increasing insulin resistance.
孕前肥胖、妊娠期糖尿病（GDM）和孕期体重增加（GWG）相互关联。这是第一项研究基因变异是否与患GDM相关，以及与GDM相关的基因变异是否与包括孕前肥胖和过度GWG在内的肥胖症相关的土耳其女性研究。
将患有GDM（n = 160）和未患有GDM（n = 145）的女性纳入病例对照研究。通过逆转录聚合酶链反应（RT-PCR）对基因（p.I27L rs1169288、p.98V rs1800574、p.S487N rs2464196）、基因（p.BsmI rs1544410、p.ApaI rs7975232、p.TaqI rs731236、p.FokI rs2228570）和基因（rs9939609）的单核苷酸多态性（SNP）进行基因分型。
基因的AA基因型与患GDM的风险增加相关（AA与AT + TT相比，24.4%对12.4%，比值比[OR] = 2.27，95%置信区间[CI][1.23 - 4.19]，p = 0.007）。基因的p.I27L GT/TT基因型与GDM风险增加相关（GT + TT与GG-野生型相比，79.4%对65.5%，OR = 2.02，95% CI [1.21 - 3.38]，p = 0.007）。然而，所有基因的SNP以及基因的p.A98V、p.S487N与患GDM均无关联（p > 0.05）。基因的AA基因型与孕前超重/肥胖风险增加相关（OR = 1.43，95% CI [1.25 - 3.4]，p = 0.035），但在调整孕前体重后与过度GWG无关联（p > 0.05）。在基因和基因携带者中，孕前体重、分娩时体重和GWG没有差异（p > 0.05）。在p.I27L TT和基因的AA基因型携带者中，胰岛素抵抗指数（HOMA-IR）和糖化血红蛋白（HbA1c）均升高（p < 0.05）。
肥胖相关基因通过对孕前肥胖的影响与GDM相关。糖尿病相关的p.I27L基因通过增加胰岛素抵抗与GDM相关。
