Gao Shanshan, Shu Shan, Wang Lijun, Zhou Juan, Yuan Zuyi
Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Nov;34(11):1589-93.
To examine the changes in the expression of pro-inflammatory and anti-inflammatory cytokines in the peripheral blood mononuclear cells (PBMCs) and elucidate the inflammatory status of apparently healthy subjects.
Peripheral blood samples (5 ml) were collected after fasting for more than 8 h from 14 healthy control subjects and 14 apparently healthy subjects with elevated serum high-sensitivity CRP (hsCRP) level (≥ 2.0 mg/L). PBMCs were separated by Ficoll density gradient centrifugation and the total RNA was extracted for real-time quantitative PCR analysis of the inflammatory cytokines, and plasma was separated for ELISA analysis of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expressions.
The gene expressions of TNF-α and MCPIP were significantly increased in PBMCs of apparently healthy subjects, while IL-6 and MCP-1 only showed slight elevations; IL-10 expression in PBMCs decreased significantly in apparently healthy subjects as compared to that in the control group. The results of ELISA showed significantly elevated TNF-α level without significant changes of plasma IL-6 level in apparently healthy subjects.
In apparently healthy subjects with normal lipid levels, chronic low-grade inflammation has occurred shown by elevated expressions of the pro-inflammatory cytokines and lowered expressions of the anti-inflammatory cytokines.
检测外周血单个核细胞（PBMCs）中促炎和抗炎细胞因子表达的变化，阐明表面健康受试者的炎症状态。
从14名健康对照受试者和14名血清高敏CRP（hsCRP）水平升高（≥2.0 mg/L）的表面健康受试者中，在禁食超过8小时后采集外周血样本（5 ml）。通过Ficoll密度梯度离心分离PBMCs，提取总RNA用于炎症细胞因子的实时定量PCR分析，并分离血浆用于白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）表达的ELISA分析。
表面健康受试者的PBMCs中TNF-α和MCPIP的基因表达显著增加，而IL-6和MCP-1仅略有升高；与对照组相比，表面健康受试者PBMCs中的IL-10表达显著降低。ELISA结果显示，表面健康受试者的TNF-α水平显著升高，而血浆IL-6水平无显著变化。
在血脂水平正常的表面健康受试者中，促炎细胞因子表达升高和抗炎细胞因子表达降低表明已发生慢性低度炎症。
