Sun Li, Deng Haohua, He Lanjie, Hu Xuemei, Huang Qi, Xue Junli, Chen Jin, Shi Xiaoli, Xu Yancheng
Department of Endocrinology Zhongnan Hospital of Wuhan University, 169# Donghu Road, Wuhan, Hubei 430071 China.
Department of Endocrinology Zhongnan Hospital of Wuhan University, 169# Donghu Road, Wuhan, Hubei 430071 China.
J Diabetes Complications. 2015 May-Jun;29(4):589-94. doi: 10.1016/j.jdiacomp.2014.12.018. Epub 2014 Dec 31.
To evaluate the expression level of NR2E1 (nuclear receptor subfamily 2,group E,member 1) and its correlation with type 2 diabetes (T2DM).
Plasma and peripheral blood mononuclear cells (PBMCs) were collected from 54 T2DM and 88 healthy individuals. The levels of free fatty acids (FFAs), total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-c), low density lipoprotein (LDL-c), fasting insulin (FIN), and fasting blood glucose (FBG) were measured. The insulin resistance index was calculated using the homeostasis model assessment (HOMA). NR2E1 in PBMCs were analyzed using real-time RT-PCR and Western blots. Tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) in the plasma were measured by enzyme-linked immunosorbent assay (ELISA). PBMCs isolated from healthy volunteers were treated with glucose or palmitate for 24h, followed by analysis for the expression level of NR2E1.The amount of TNF-α and IL-6 secreted into the supernatant were measured by ELISA.
FIN, FBG, HOMA and TNF-α, IL-6 were significantly higher in diabetic patients, compared to the control group. Levels of NR2E1 were significantly higher in the PBMCs isolated from the diabetic group, compared to the control group. NR2E1 expression was positively correlated with FBG, FIN, HOMA, FFAs, TNF-α and IL-6. Glucose and palmitate treatment significantly increased NR2E1 gene expression and inflammatory cytokines production in PBMCs in vitro.
Increased NR2E1 level may be closely associated with inflammation and disorder of lipid and glucose metabolism in diabetic patients.
评估核受体亚家族2组E成员1（NR2E1）的表达水平及其与2型糖尿病（T2DM）的相关性。
收集54例T2DM患者和88例健康个体的血浆及外周血单个核细胞（PBMC）。检测游离脂肪酸（FFA）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-c）、低密度脂蛋白（LDL-c）、空腹胰岛素（FIN）和空腹血糖（FBG）水平。采用稳态模型评估（HOMA）计算胰岛素抵抗指数。使用实时RT-PCR和蛋白质免疫印迹法分析PBMC中的NR2E1。采用酶联免疫吸附测定（ELISA）法检测血浆中的肿瘤坏死因子α（TNF-α）和白细胞介素-6（IL-6）。将从健康志愿者分离的PBMC用葡萄糖或棕榈酸处理24小时，随后分析NR2E1的表达水平。通过ELISA法检测上清液中分泌的TNF-α和IL-6的量。
与对照组相比，糖尿病患者的FIN、FBG、HOMA以及TNF-α、IL-6显著更高。与对照组相比，从糖尿病组分离的PBMC中NR2E1水平显著更高。NR2E1表达与FBG、FIN、HOMA、FFA、TNF-α和IL-6呈正相关。葡萄糖和棕榈酸处理显著增加体外PBMC中NR2E1基因表达和炎性细胞因子的产生。
NR2E1水平升高可能与糖尿病患者的炎症以及脂质和葡萄糖代谢紊乱密切相关。
